Počet záznamů: 1
Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels
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SYSNO ASEP 0562009 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Absolute Counting Method with Multiplexing Capability for Estimating the Number Concentration of Nanoparticles Using Anisotropically Collapsed Gels Tvůrce(i) Hlaváček, Antonín (UIACH-O) ORCID
Křivánková, Jana (UIACH-O) RID, ORCID
Brožková, Hana (UIACH-O)
Weisová, Julie (UIACH-O)
Pizúrová, Naděžda (UFM-A) RID, ORCID
Foret, František (UIACH-O) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 94, č. 41 (2022), s. 14340-14348Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova absolute quantification ; counting ; photon-upconversion ; fluorescence ; nanoparticles Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Vědní obor RIV – spolupráce Ústav fyziky materiálu - Analytická chemie, separace CEP GA21-03156S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 ; UFM-A - RVO:68081723 UT WOS 000870033800001 EID SCOPUS 85139477673 DOI 10.1021/acs.analchem.2c02989 Anotace The presented method is suitable for estimating the number concentration of nanoparticles and their bioconjugates. The method benefits from well-defined immobilization of nanomaterials in anisotropically collapsed agarose gel. Once immobilized, the nanoparticles are imaged by a microscope and counted. The number of counted nanoparticles is then used for estimating the number concentration. The accuracy and precision of the method were characterized by the samples of photon-upconversion nanoparticles. By analyzing the brightness of the emission from single diffraction-limited spots, it was possible to analyze also the content of UCNP clusters. The detection of extremely weak emitters was proved. The method supports several microscope imaging modalities such as photon-upconversion, fluorescence, dark-field, and bright-field microscopy. The applicability of these modalities is demonstrated by imaging UCNPs, dye-doped fluorescent silica nanoparticles, CdSe/ZnS quantum dots, and submicron silica particles. Thus, the method is not limited to a particular nanomaterial or imaging modality type. The method was developed for aqueous dispersions of nanoparticles and utilized the agarose gel to provide a well-defined immobilization of nanomaterials. However, the gels are not limited to only agarose or aqueous dispersions. Therefore, the method can be likely extended to other types of solvents and gel matrices. Interestingly, agarose contains only light elements such as carbon, oxygen, hydrogen, and a small amount of sulfur. Therefore, the method is potentially compatible with TEM, which can provide an even more detailed analysis. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2023 Elektronická adresa https://hdl.handle.net/11104/0334437
Počet záznamů: 1