Počet záznamů: 1
Bacterial Aryl Sulfotransferases in Selective and Sustainable Sulfation of Biologically Active Compounds using Novel Sulfate Donors
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SYSNO ASEP 0561642 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bacterial Aryl Sulfotransferases in Selective and Sustainable Sulfation of Biologically Active Compounds using Novel Sulfate Donors Tvůrce(i) Brodsky, Katerina (MBU-M) ORCID
Káňová, Kristýna (MBU-M) ORCID
Konvalinková, Dorota (MBU-M)
Slámová, Kristýna (MBU-M) RID, ORCID
Pelantová, Helena (MBU-M) ORCID, RID
Valentová, Kateřina (MBU-M) RID, ORCID
Bojarová, Pavla (MBU-M) ORCID
Křen, Vladimír (MBU-M) RID, ORCID
Petrásková, Lucie (MBU-M) ORCIDČíslo článku e202201253 Zdroj.dok. ChemSusChem. - : Wiley - ISSN 1864-5631
Roč. 15, č. 18 (2022)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova aryl sulfotransferase ; biocatalysis ; enzyme catalysis ; leaving group ; sulfation Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA19-00043S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000831332600001 EID SCOPUS 85135030849 DOI 10.1002/cssc.202201253 Anotace Regioselective sulfation of bioactive compounds is a vital and scarcely studied topic in enzyme-catalyzed transformations and metabolomics. The major bottleneck of enzymatic sulfation consists in finding suitable sulfate donors. In this regard, 3 '-phosphoadenosine 5 '-phosphosulfate (PAPS)-independent aryl sulfotransferases using aromatic sulfate donors are a favored choice due to their cost-effectiveness. This work presents a unique study of five sulfate donors differing in their leaving group pK(a) values with a new His-tagged construct of aryl sulfotransferase from Desulfitobacterium hafniense (DhAST-tag). DhAST-tag was purified to homogeneity and biochemically characterized. Two new donors (3-nitrophenyl sulfate and 2-nitrophenyl sulfate) were synthesized. The kinetic parameters of these and other commercial sulfates (4-nitrophenyl, 4-methylumbelliferyl, and phenyl) revealed large differences with respect to the structure of the leaving group. These donors were screened for the sulfation of selected flavonoids (myricetin, chrysin) and phenolic acids (gallate, 3,4-dihydroxyphenylacetate). The donor impact on the sulfation regioselectivity and yield was assessed. The obtained regioselectively sulfated compounds are authentic human metabolites required as standards in clinical trials. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cssc.202201253
Počet záznamů: 1