Počet záznamů: 1  

The Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae

  1. 1.
    SYSNO ASEP0560767
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevThe Sixth Element: a 102-kb RepABC Plasmid of Xenologous Origin Modulates Chromosomal Gene Expression in Dinoroseobacter shibae
    Tvůrce(i) Koppenhofer, S. (CA)
    Tomasch, Jurgen (MBU-M) ORCID, RID
    Ringel, V. (DE)
    Birmes, L. (DE)
    Brinkmann, H. (DE)
    Sproeer, C. (DE)
    Jarek, M. (DE)
    Wang, H. (DE)
    Pradella, S. (DE)
    Wagner-Doebler, I. (DE)
    Petersen, J. (DE)
    Číslo článku00264-22
    Zdroj.dok.mSystems. - : American Society for Microbiology - ISSN 2379-5077
    Roč. 7, č. 4 (2022)
    Poč.str.15 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaRoseobacteraceae ; plasmid stability ; transcriptomics ; denitrification ; heavy metal resistance ; CtrA regulon
    Vědní obor RIVEE - Mikrobiologie, virologie
    Obor OECDMicrobiology
    Způsob publikováníOpen access
    Institucionální podporaMBU-M - RVO:61388971
    UT WOS000836331300001
    EID SCOPUS85137038605
    DOI10.1128/msystems.00264-22
    AnotaceThe model organism Dinoroseobacter shibae and many other marine Rhodobacterales (Roseobacteraceae, Aiphaproteobacteria) are characterized by a multipartite genome organization. Here, we show that the original isolate (Dshi-6) contained six extrachromosomal replicons (ECRs), whereas the strain deposited at the DSMZ (Dshi-5) lacked a 102-kb plasmid. To determine the role of the sixth plasmid, we investigated the genomic and physiological differences between the two strains. Therefore, both genomes were (re)sequenced, and gene expression, growth, and substrate utilization were examined. For comparison, we included additional plasmid-cured strains in the analysis. In the Dshi-6 population, the conjugative 102-kb RepABC-9 plasmid was present in only about 50% of the cells, irrespective of its experimentally validated stability. In the presence of the sixth plasmid, copy number changes of other ECRs, in particular, a decrease of the 86-kb plasmid, were observed. The most conspicuous finding was the strong influence of plasmids on chromosomal gene expression, especially the repression of the CtrA regulon and the activation of the denitrification gene cluster. Expression is inversely controlled by either the presence of the 102-kb plasmid or the absence of the 86-kb plasmid. We identified regulatory genes on both plasmids, i.e., a sigma 70 factor and a quorum sensing synthase, that might be responsible for these major changes. The tremendous effects that were probably even underestimated challenge the current understanding of the relevance of volatile plasmids not only for the original host but also for new recipients after conjugation.
    PracovištěMikrobiologický ústav
    KontaktEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Rok sběru2023
    Elektronická adresahttps://journals.asm.org/doi/epub/10.1128/msystems.00264-22
Počet záznamů: 1  

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