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Antibiotic-loaded amphiphilic chitosan nanoparticles target macrophages and kill an intracellular pathogen
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SYSNO ASEP 0559145 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antibiotic-loaded amphiphilic chitosan nanoparticles target macrophages and kill an intracellular pathogen Tvůrce(i) Trousil, Jiří (UMCH-V) RID, ORCID
Dal, N.-J. K. (NO)
Fenaroli, F. (NO)
Schlachet, I. (IL)
Kubíčková, P. (CZ)
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Pavlova, Ewa (UMCH-V) RID
Škorič, M. (CZ)
Trejbalová, Kateřina (UMG-J) RID
Pavliš, O. (CZ)
Sosnik, A. (IL)Číslo článku 2201853 Zdroj.dok. Small. - : Wiley - ISSN 1613-6810
Roč. 18, č. 28 (2022)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova amphiphilic chitosan nanoparticles ; intracellular infections ; levofloxacin Obor OECD Biomaterials (as related to medical implants, devices, sensors) Vědní obor RIV – spolupráce Ústav makromolekulární chemie - Biotechnologie a bionika
Ústav molekulární genetiky - Mikrobiologie, virologieZpůsob publikování Omezený přístup Institucionální podpora UMCH-V - RVO:61389013 ; UMG-J - RVO:68378050 UT WOS 000809630500001 EID SCOPUS 85131720748 DOI 10.1002/smll.202201853 Anotace In this work, levofloxacin (LVX), a third-generation fluoroquinolone antibiotic, is encapsulated within amphiphilic polymeric nanoparticles of a chitosan-g-poly(methyl methacrylate) produced by self-assembly and physically stabilized by ionotropic crosslinking with sodium tripolyphosphate. Non-crosslinked nanoparticles display a size of 29 nm and a zeta-potential of +36 mV, while the crosslinked counterparts display 45 nm and +24 mV, respectively. The cell compatibility, uptake, and intracellular trafficking are characterized in the murine alveolar macrophage cell line MH-S and the human bronchial epithelial cell line BEAS-2B in vitro. Internalization events are detected after 10 min and the uptake is inhibited by several endocytosis inhibitors, indicating the involvement of complex endocytic pathways. In addition, the nanoparticles are detected in the lysosomal compartment. Then, the antibacterial efficacy of LVX-loaded nanoformulations (50% w/w drug content) is assessed in MH-S and BEAS-2B cells infected with Staphylococcus aureus and the bacterial burden is decreased by 49% and 46%, respectively. In contrast, free LVX leads to a decrease of 8% and 5%, respectively, in the same infected cell lines. Finally, intravenous injection to a zebrafish larval model shows that the nanoparticles accumulate in macrophages and endothelium and demonstrate the promise of these amphiphilic nanoparticles to target intracellular infections.
Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2023 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1002/smll.202201853
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