Počet záznamů: 1
Evaluation of two artificial infection methods of live ticks as tools for studying interactions between tick-borne viruses and their tick vectors
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SYSNO ASEP 0557058 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Evaluation of two artificial infection methods of live ticks as tools for studying interactions between tick-borne viruses and their tick vectors Tvůrce(i) Migne, C. (FR)
Hönig, Václav (BC-A) RID, ORCID
Bonnet, S. (FR)
Palus, Martin (BC-A) RID, ORCID
Rakotobe, S. (FR)
Galon, C. (FR)
Heckmann, A. (FR)
Výletová, Eva (BC-A)
Devillers, E. (FR)
Attoui, H. (FR)
Růžek, Daniel (BC-A) RID, ORCID
Moutailler, S. (FR)Celkový počet autorů 12 Číslo článku 491 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Poč.str. 13 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova ixodes-persulcatus ticks ; encephalitis-virus ; kemerovo virus ; ricinus ticks ; aedes-aegypti ; transmission ; mice ; competence ; variants ; survival Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA20-14325S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000783767400019 EID SCOPUS 85122788698 DOI 10.1038/s41598-021-04498-9 Anotace Up to 170 tick-borne viruses (TBVs) have been identified to date. However, there is a paucity of information regarding TBVs and their interaction with respective vectors, limiting the development of new effective and urgently needed control methods. To overcome this gap of knowledge, it is essential to reproduce transmission cycles under controlled laboratory conditions. In this study we assessed an artificial feeding system (AFS) and an immersion technique (IT) to infect Ixodes ricinus ticks with tick-borne encephalitis (TBE) and Kemerovo (KEM) virus, both known to be transmitted predominantly by ixodid ticks. Both methods permitted TBEV acquisition by ticks and we further confirmed virus trans-stadial transmission and onward transmission to a vertebrate host. However, only artificial feeding system allowed to demonstrate both acquisition by ticks and trans-stadial transmission for KEMV. Yet we did not observe transmission of KEMV to mice (IFNAR(-/-) or BALB/c). Artificial infection methods of ticks are important tools to study tick-virus interactions. When optimally used under laboratory settings, they provide important insights into tick-borne virus transmission cycles. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2023 Elektronická adresa https://www.nature.com/articles/s41598-021-04498-9
Počet záznamů: 1