Počet záznamů: 1
DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes
- 1.
SYSNO ASEP 0556437 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes Tvůrce(i) Scholz, P. (DE)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Fernkorn, M. (DE)
Škrabálková, Eliška (UEB-Q) ORCID
Pleskot, Roman (UEB-Q) RID, ORCID
Blersch, K. (DE)
Munnik, T. (NL)
Potocký, Martin (UEB-Q) RID, ORCID
Ischebeck, T. (DE)Celkový počet autorů 9 Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 233, č. 5 (2022), s. 2185-2202Poč.str. 18 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova diacylglycerol kinase ; lipid signaling ; pectin Obor OECD Cell biology CEP GA17-27477S GA ČR - Grantová agentura ČR GA20-21547S GA ČR - Grantová agentura ČR LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000740760700001 EID SCOPUS 85122680384 DOI 10.1111/nph.17930 Anotace Pollen tubes require a tightly regulated pectin secretion machinery to sustain the cell wall plasticity required for polar tip growth. Involved in this regulation at the apical plasma membrane are proteins and signaling molecules, including phosphoinositides and phosphatidic acid (PA). However, the contribution of diacylglycerol kinases (DGKs) is not clear. We transiently expressed tobacco DGKs in pollen tubes to identify a plasma membrane (PM)-localized isoform, and then to study its effect on pollen tube growth, pectin secretion and lipid signaling. In order to potentially downregulate DGK5 function, we overexpressed an inactive variant. Only one of eight DGKs displayed a confined localization at the apical PM. We could demonstrate its enzymatic activity and that a kinase-dead variant was inactive. Overexpression of either variant led to differential perturbations including misregulation of pectin secretion. One mode of regulation could be that DGK5-formed PA regulates phosphatidylinositol 4-phosphate 5-kinases, as overexpression of the inactive DGK5 variant not only led to a reduction of PA but also of phosphatidylinositol 4,5-bisphosphate levels and suppressed related growth phenotypes. We conclude that DGK5 is an additional player of polar tip growth that regulates pectin secretion probably in a common pathway with PI4P 5-kinases. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2023 Elektronická adresa http://doi.org/10.1111/nph.17930
Počet záznamů: 1