Počet záznamů: 1
Topological Analysis of gamma H2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair
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SYSNO ASEP 0554412 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Topological Analysis of gamma H2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair Tvůrce(i) Hahn, H. (DE)
Neitzel, Ch. (DE)
Kopečná, Olga (BFU-R) ORCID
Heermann, D.W. (DE)
Falk, Martin (BFU-R) RID, ORCID
Hausmann, M. (DE)Celkový počet autorů 6 Číslo článku 5561 Zdroj.dok. Cancers (Basel). - : MDPI
Roč. 13, č. 21 (2021)Poč.str. 21 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova histone h2ax phosphorylation ; complex cell responses ; homologous recombination ; nanoscopy techniques ; pathway choice Vědní obor RIV FD - Onkologie a hematologie Obor OECD Oncology CEP GC20-04109J GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000718725500001 DOI 10.3390/cancers13215561 Anotace Simple Summary: Super-resolution single-molecule localization microscopy (SMLM) allows the detection of cluster formation of phosphorylation sites and recruited repair proteins (e.g., MRE11) around DNA double-strand breaks induced by ionizing radiation. The spatial arrangement of these targets can be described by distance frequency distributions, which can be used as a measure for cluster size and the ratio of cluster numbers per specimen analyzed. For the analysis of the shape of clusters and their topology and similarity, persistent homology was applied and the features were compared along the DNA repair process for two different cell lines (MCF-7 breast cancer cells and CCD-1059SK human skin fibroblasts). The similarity values for components and holes were averaged for each repair time point post-irradiation so that it was possible to compare not only clusters with each other but also cell specimens of different repair periods with each other. It was shown that after X-ray irradiation, these similarity values were high for gamma H2AX clusters in the early repair phase, while MRE11 clusters revealed an increased similarity at later time points. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2072-6694/13/21/5561
Počet záznamů: 1