Počet záznamů: 1
A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface
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SYSNO ASEP 0554407 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface Tvůrce(i) Hesko, Ondřej (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Špaček, Jan (BFU-R) ORCIDCelkový počet autorů 3 Číslo článku 115773 Zdroj.dok. Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 901, NOV 15 2021 (2021)Poč.str. 6 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova electrochemical oxidation ; nucleic-acids ; oligonucleotides ; dna ; voltammetry Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA20-03187S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora BFU-R - RVO:68081707 UT WOS 000714137900001 EID SCOPUS 85117389455 DOI 10.1016/j.jelechem.2021.115773 Anotace Previously it has been shown that cyclic nucleoside monophosphates can spontaneously polymerize to form RNA oligonucleotides under conditions simulating prebiotic conditions on Archean Earth. The most efficient polymerization was documented with 3',5'-cyclic guanosine monophosphate (cGMP). In this work a method for fast detection of short polyG RNAs present in a large overabundance of cGMP, modeling conditions in the non-enzymatic nucleotide polymerization mixtures, is presented. The method is based on electrochemical measurements of guanine (G) oxidation signals yielded by RNA oligomers adsorbed onto the surface of a pyrolytic graphite electrode (PGE). To avoid false positive results arising from the G oxidation signals due to coadsorbed cGMP, a method for selective removal of the monomers from the electrode surface has been devised. In the first step, both cGMP and RNAs are co-adsorbed onto the PGE surface. In the second step, the cGMP is selectively desorbed using treatments in solutions of different tested surfactants (SDS, Tween 20 or Triton X100), or by washing in deionized water at elevated temperature. We show that this new approach is suitable for selective analysis of products of polymerization reactions from mixtures of their building blocks. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1572665721007992?via%3Dihub
Počet záznamů: 1