Počet záznamů: 1  

Caspase Inhibition Affects the Expression of Autophagy-Related Molecules in Chondrocytes

  1. 1.
    SYSNO ASEP0553617
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevCaspase Inhibition Affects the Expression of Autophagy-Related Molecules in Chondrocytes
    Tvůrce(i) Veselá, Barbora (UZFG-Y) RID
    Švandová, Eva (UZFG-Y) RID, ORCID
    Ramešová, Alice (UZFG-Y)
    Kratochvílová, Adéla (UZFG-Y) ORCID
    Tucker, A. S. (GB)
    Matalová, Eva (UZFG-Y) RID
    Zdroj.dok.Cartilage . - : Sage - ISSN 1947-6035
    Roč. 13, 2 Suppl (2021), 956S-968S
    Poč.str.13 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaautophagy ; caspase ; gene expression
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDCell biology
    CEPGA19-12023S GA ČR - Grantová agentura ČR
    LTC18081 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
    Způsob publikováníOmezený přístup
    Institucionální podporaUZFG-Y - RVO:67985904
    UT WOS000545369100001
    EID SCOPUS85087462404
    DOI10.1177/1947603520938444
    AnotaceObjective. Caspases, cysteine proteases traditionally associated with apoptosis and inflammation, have recently been identified as important regulators of autophagy and reported within the growth plate, a cartilaginous part of the developing bone. The aim of this research was to identify novel autophagy-related molecules affected by inhibition of pro-apoptotic caspases in chondrocytes.Design. Chondrocyte micromasses derived from mouse limb buds were treated with pharmacological inhibitors of caspases. Autophagy-related gene expression was examined and possible novel molecules were confirmed by real-time polymerase chain reaction and immunocytofluorescence. Individual caspases inhibitors were used to identify the effect of specific caspases.Results. Chondrogenesis accompanied by caspase activation and autophagy progression was confirmed in micromass cultures. Expression of several autophagy-associated genes was significantly altered in the caspases inhibitors treated groups with the most prominent decrease for Pik3cg and increase of Tnfsf10. The results showed the specific pro-apoptotic caspases that play a role in these effects. Importantly, use of caspase inhibitors mimicked changes triggered by an autophagy stimulator, rapamycin, linking loss of caspase activity to an increase in autophagy.Conclusion. Caspase inhibition significantly affects regulation of autophagy-related genes in chondrocytes cultures. Detected markers are of importance in diagnostics and thus the data presented here open new perspectives in the field of cartilage development and degradation.
    PracovištěÚstav živočišné fyziologie a genetiky
    KontaktJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Rok sběru2022
    Elektronická adresahttps://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=14298856883
Počet záznamů: 1  

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