Počet záznamů: 1
New Insights into Tolytoxin Effect in Human Cancer Cells: Apoptosis Induction and the Relevance of Hydroxyl Substitution of Its Macrolide Cycle on Compound Potency
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SYSNO ASEP 0553487 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název New Insights into Tolytoxin Effect in Human Cancer Cells: Apoptosis Induction and the Relevance of Hydroxyl Substitution of Its Macrolide Cycle on Compound Potency Tvůrce(i) Delawská, Kateřina (MBU-M) ORCID
Divoká, Petra (MBU-M)
Sedlák, David (UMG-J) RID
Kuzma, Marek (MBU-M) ORCID, RID
Saurav, Kumar (MBU-M) ORCID
Macho, Markéta (MBU-M) ORCID
Steinbach, G. (HU)
Hrouzek, Pavel (MBU-M) ORCIDČíslo článku e202100489 Zdroj.dok. Chembiochem. - : Wiley - ISSN 1439-4227
Roč. 23, č. 1 (2022)Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova actin ; binding ; scytophycins ; agents ; actin disruption ; apoptosis ; cancer ; scytophycines ; tolytoxin Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Vědní obor RIV – spolupráce Ústav molekulární genetiky CEP GF21-05649K GA ČR - Grantová agentura ČR LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018130 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 UT WOS 000728404200001 EID SCOPUS 85120791599 DOI 10.1002/cbic.202100489 Anotace Scytophycins, including tolytoxin, represent a class of actin disrupting macrolides with strong antiproliferative effects on human cells. Despite intense research, little attention has been paid to scytophycin-induced cell death or the structural features affecting its potency. We show that tolytoxin and its natural analogue, 7-O-methylscytophycin B, lacking the hydroxyl substitution in its macrolactone ring, differ substantially in their cytotoxic effect. Both compounds increase the level of caspases 3/7, which are the main executioner proteases during apoptosis, in HeLa wild-type (WT) cells. However, no caspase activity was detected in HeLa cells lacking Bax/Bak proteins crucial for caspase activation via the mitochondrial pathway. Obtained data strongly suggests that scytophycins are capable of inducing mitochondria-dependent apoptosis. These findings encourage further research in structure-activity relationships in scytophycins and highlight the potential of these compounds in targeted drug delivery. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/cbic.202100489
Počet záznamů: 1