Počet záznamů: 1
The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote
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SYSNO ASEP 0543232 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote Tvůrce(i) Vítová, Milada (MBU-M) RID, ORCID
Lanta, Vojtěch (MBU-M)
Čížková, Mária (MBU-M) ORCID
Jakubec, M. (NO)
Rise, F. (NO)
Halskau, O. (NO)
Bišová, Kateřina (MBU-M) RID
Furse, S. (NO)Číslo článku 158965 Zdroj.dok. Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. - : Elsevier - ISSN 1388-1981
Roč. 1866, č. 8 (2021)Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova phase-transitions ; chlamydomonas-reinhardtii ; dependent kinases ; cleavage furrow ; green-algae ; yeast ; lipids ; proteins ; phosphatidylethanolamine ; redistribution ; Cell cycle ; Cell division ; Cell structure ; Desmodesmus quadricauda ; Green algae ; Lipid composition ; Lipid metabolism Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000656122900009 EID SCOPUS 85107319643 DOI 10.1016/j.bbalip.2021.158965 Anotace The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. We tested the hypothesis that the biosynthesis of structural lipids is linked to the cell cycle. If true, this would suggest that the cell's structure is important for progress through and perhaps even control of the cell cycle. Lipidomics (P-31 NMR and MS), proteomics (Western immunoblotting) and transcriptomics (RT-qPCR) techniques were used to profile the lipid fraction and characterise aspects of its metabolism at seven stages of the cell cycle of the model eukaryote, Desmodesmus quadricauda. We found considerable, transient increases in the abundance of phosphatidylethanolamine during the G(1) phase (+35%, ethanolamine phosphate cytidylyltransferase increased 2.5x) and phosphatidylglycerol (+100%, phosphatidylglycerol synthase increased 22x) over the G1/pre-replication phase boundary. The relative abundance of phosphatidylcholine fell by similar to 35% during the G1. N-Methyl transferases for the conversion of phosphatidylethanolamine into phosphatidylcholine were not found in the de novo transcriptome profile, though a choline phosphate transferase was found, suggesting that the Kennedy pathway is the principal route for the synthesis of PC. The fatty acid profiles of the four most abundant lipids suggested that these lipids were not generally converted between one another. This study shows for the first time that there are considerable changes in the biosynthesis of the three most abundant phospholipid classes in the normal cell cycle of D. quadricauda, by margins large enough to elicit changes to the physical properties of membranes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S1388198121000937?via%3Dihub
Počet záznamů: 1