Počet záznamů: 1
Insight into formation propensity of pseudocircular DNA G-hairpins
- 1.
SYSNO ASEP 0542802 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Insight into formation propensity of pseudocircular DNA G-hairpins Tvůrce(i) Zivkovic, M.L. (SI)
Gajarský, M. (CZ)
Bekova, K. (CZ)
Stadlbauer, Petr (BFU-R) ORCID
Vicherek, L. (CZ)
Petrová, Magdalena (UOCHB-X) RID
Fiala, R. (CZ)
Rosenberg, Ivan (UOCHB-X) RID, ORCID
Šponer, Jiří (BFU-R) RID, ORCID
Plavec, J. (SI)
Trantírek, L. (CZ)Celkový počet autorů 11 Zdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 49, č. 4 (2021), s. 2317-2332Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova molecular-dynamics simulations ; g-quadruplex structure ; amber force-field ; g-triplex ; folding pathways Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Vědní obor RIV – spolupráce Ústav organické chemie a biochemie - Biochemie CEP GA17-12703S GA ČR - Grantová agentura ČR EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 ; UOCHB-X - RVO:61388963 UT WOS 000637321900039 EID SCOPUS 85102407851 DOI 10.1093/nar/gkab029 Anotace We recently showed that Saccharomyces cerevisiae telomeric DNA can fold into an unprecedented pseudocircular G-hairpin (PGH) structure. However, the formation of PGHs in the context of extended sequences, which is a prerequisite for their function in vivo and their applications in biotechnology, has not been elucidated. Here, we show that despite its 'circular' nature, PGHs tolerate single-stranded (ss) protrusions. High-resolution NMR structure of a novel member of PGH family reveals the atomistic details on a junction between ssDNA and PGH unit. Identification of new sequences capable of folding into one of the two forms of PGH helped in defining minimal sequence requirements for their formation. Our time-resolved NMR data indicate a possibility that PGHs fold via a complex kinetic partitioning mechanism and suggests the existence of K+ ion-dependent PGH folding intermediates. The data not only provide an explanation of cation-type-dependent formation of PGHs, but also explain the unusually large hysteresis between PGH melting and annealing noted in our previous study. Our findings have important implications for DNA biology and nanotechnology. Overrepresentation of sequences able to form PGHs in the evolutionary-conserved regions of the human genome implies their functionally important biological role(s). Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://academic.oup.com/nar/article/49/4/2317/6125665
Počet záznamů: 1