Počet záznamů: 1
Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming
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SYSNO ASEP 0542170 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming Tvůrce(i) Levoux, J. (FR)
Prola, A. (FR)
Lafuste, P. (FR)
Gervais, M. (FR)
Chevallier, N. (FR)
Koumaiha, Z. (FR)
Kefi, K. (FR)
Braud, L. (FR)
Schmitt, A. (FR)
Yacia, A. (FR)
Schirmann, A. (FR)
Hersant, B. (FR)
Sid-Ahmed, M. (FR)
Ben Larbi, S. (FR)
Komrsková, Kateřina (BTO-N) ORCID
Rohlena, Jakub (BTO-N) RID, ORCID
Relaix, F. (FR)
Neužil, Jiří (BTO-N) RID
Rodriguez, A.-M. (FR)Celkový počet autorů 19 Zdroj.dok. Cell Metabolism. - : Cell Press - ISSN 1550-4131
Roč. 33, č. 2 (2021), s. 283-299Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova angiogenesis ; cell therapy ; de novo ; fatty acid synthesis ; citrate Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP GA20-18513S GA ČR - Grantová agentura ČR GA18-10832S GA ČR - Grantová agentura ČR GA20-05942S GA ČR - Grantová agentura ČR NU20-03-00309 GA MZd - Ministerstvo zdravotnictví NV17-30138A GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000632624500009 EID SCOPUS 85099679718 DOI 10.1016/j.cmet.2020.12.006 Anotace Platelets are known to enhance the wound-healing activity of mesenchymal stem cells (MSCs). However, the mechanism by which platelets improve the therapeutic potential of MSCs has not been elucidated. Here, we provide evidence that, upon their activation, platelets transfer respiratory-competent mitochondria to MSCs primarily via dynamin-dependent clathrin-mediated endocytosis. We found that this process enhances the therapeutic efficacy of MSCs following their engraftment in several mouse models of tissue injury, including full-thickness cutaneous wound and dystrophic skeletal muscle. By combining in vitro and in vivo experiments, we demonstrate that platelet-derived mitochondria promote the pro-angiogenic activity of MSCs via their metabolic remodeling. Notably, we show that activation of the de novo fatty acid synthesis pathway is required for increased secretion of pro-angiogenic factors by platelet-preconditioned MSCs. These results reveal a new mechanism by which platelets potentiate MSC properties and underline the importance of testing platelet mitochondria quality prior to their clinical use. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2022 Elektronická adresa https://www.sciencedirect.com/science/article/abs/pii/S1550413120306616?via%3Dihub
Počet záznamů: 1