Počet záznamů: 1
Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142
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SYSNO ASEP 0541527 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142 Tvůrce(i) Polerecký, L. (CZ)
Masuda, Takako (MBU-M) ORCID
Eichner, Meri (MBU-M) ORCID, RID
Rabouille, S. (FR)
Vancová, Marie (BC-A) RID, ORCID
Kienhuis, M. V. M. (NL)
Bernát, Gábor (MBU-M) ORCID
Bonomi-Barufi, J. (BR)
Campbell, D. A. (CA)
Claquin, P. (FR)
Červený, Jan (UEK-B) RID, ORCID, SAI
Giordano, Mario (MBU-M) ORCID
Kotabová, Eva (MBU-M) RID, ORCID
Kromkamp, J. (NL)
Lombardi, A. T. (BR)
Lukeš, Martin (MBU-M) ORCID
Prášil, Ondřej (MBU-M) RID, ORCID
Stephan, S. (DE)
Suggett, D. (AU)
Zavřel, Tomáš (UEK-B) RID, SAI, ORCID
Halsey, K. H. (US)Číslo článku 620915 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 12, FEB 4 (2021)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Crocosphaera subtropica ; Cyanothece ; photosynthesis ; carbon fixation ; nitrogen fixation ; nanoSIMS ; tem Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Vědní obor RIV – spolupráce Biologické centrum (od r. 2006) - Genetika a molekulární biologie
Ústav výzkumu globální změny - Mikrobiologie, virologieCEP LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_026/0008413 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA18-24397S GA ČR - Grantová agentura ČR GA20-17627S GA ČR - Grantová agentura ČR GJ20-02827Y GA ČR - Grantová agentura ČR Výzkumná infrastruktura Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; BC-A - RVO:60077344 ; UEK-B - RVO:86652079 UT WOS 000619068700001 EID SCOPUS 85101223677 DOI 10.3389/fmicb.2021.620915 Anotace Unicellular nitrogen fixing cyanobacteria (UCYN) are abundant members of phytoplankton communities in a wide range of marine environments, including those with rapidly changing nitrogen (N) concentrations. We hypothesized that differences in N availability (N-2 vs. combined N) would cause UCYN to shift strategies of intracellular N and C allocation. We used transmission electron microscopy and nanoscale secondary ion mass spectrometry imaging to track assimilation and intracellular allocation of C-13-labeled CO2 and N-15-labeled N-2 or NO3 at different periods across a diel cycle in Cyanothece sp. ATCC 51142. We present new ideas on interpreting these imaging data, including the influences of pre-incubation cellular C and N contents and turnover rates of inclusion bodies. Within cultures growing diazotrophically, distinct subpopulations were detected that fixed N-2 at night or in the morning. Additional significant within-population heterogeneity was likely caused by differences in the relative amounts of N assimilated into cyanophycin from sources external and internal to the cells. Whether growing on N-2 or NO3, cells prioritized cyanophycin synthesis when N assimilation rates were highest. N assimilation in cells growing on NO3 switched from cyanophycin synthesis to protein synthesis, suggesting that once a cyanophycin quota is met, it is bypassed in favor of protein synthesis. Growth on NO3 also revealed that at night, there is a very low level of CO2 assimilation into polysaccharides simultaneous with their catabolism for protein synthesis. This study revealed multiple, detailed mechanisms underlying C and N management in Cyanothece that facilitate its success in dynamic aquatic environments. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2021.620915/full
Počet záznamů: 1