Počet záznamů: 1  

Peptide-Based Identification of Phytophthora Isolates and Phytophthora Detection in Planta

  1. 1.
    0540057 - BFÚ 2021 RIV CH eng J - Článek v odborném periodiku
    Berka, M. - Greplová, M. - Saiz-Fernández, I. - Novák, J. - Luklová, M. - Zelená, P. - Tomšovský, M. - Brzobohatý, Břetislav - Černý, M.
    Peptide-Based Identification of Phytophthora Isolates and Phytophthora Detection in Planta.
    International Journal of Molecular Sciences. Roč. 21, č. 24 (2020), č. článku 9463. E-ISSN 1422-0067
    Institucionální podpora: RVO:68081707
    Klíčová slova: infection * infestans * Phytophthora * proteomics * palmivora
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 5.924, rok: 2020
    Způsob publikování: Open access
    https://www.mdpi.com/1422-0067/21/24/9463

    Phytophthora is arguably one of the most damaging genera of plant pathogens. This pathogen is well suited to transmission via the international plant trade, and globalization has been promoting its spread since the 19th century. Early detection is essential for reducing its economic and ecological impact. Here, a shotgun proteomics approach was utilized for Phytophthora analysis. The collection of 37 Phytophthora isolates representing 12 different species was screened for species-specific peptide patterns. Next, Phytophthora proteins were detected in planta, employing model plants Solanum tuberosum and Hordeum vulgare. Although the evolutionarily conserved sequences represented more than 10% of the host proteome and limited the pathogen detection, the comparison between qPCR and protein data highlighted more than 300 protein markers, which correlated positively with the amount of P. infestans DNA. Finally, the analysis of P. palmivora response in barley revealed significant alterations in plant metabolism. These changes included enzymes of cell wall metabolism, ROS production, and proteins involved in trafficking. The observed root-specific attenuation in stress-response mechanisms, including the biosynthesis of jasmonates, ethylene and polyamines, and an accumulation of serotonin, provided the first insight into molecular mechanisms behind this particular biotic interaction.
    Trvalý link: http://hdl.handle.net/11104/0317734

     
     
Počet záznamů: 1  

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