Počet záznamů: 1
Impact of parasitic lifestyle and different types of centromere organization on chromosome and genome evolution in the plant genus Cuscuta
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SYSNO ASEP 0535085 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Impact of parasitic lifestyle and different types of centromere organization on chromosome and genome evolution in the plant genus Cuscuta Tvůrce(i) Neumann, Pavel (BC-A) RID, ORCID
Oliveira, Ludmila (BC-A) RID, ORCID
Čížková, Jana (UEB-Q) RID, ORCID
Jang, Tae-Soo (BC-A) ORCID
Klemme, Sonja (BC-A)
Novák, Petr (BC-A) RID, ORCID
Stelmach, K. (PL)
Koblížková, Andrea (BC-A)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Macas, Jiří (BC-A) RID, ORCID, SAICelkový počet autorů 10 Zdroj.dok. New Phytologist - ISSN 0028-646X
Roč. 229, č. 4 (2021), s. 2365-2377Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova centromere ; Convolvulaceae ; Cuscuta ; genome size ; holocentric chromosomes ; monocentric chromosomes ; repetitive DNA Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) Vědní obor RIV – spolupráce Ústav experimentální botaniky - Genetika a molekulární biologie CEP GA17-09750S GA ČR - Grantová agentura ČR EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 ; UEB-Q - RVO:61389030 UT WOS 000588834000001 EID SCOPUS 85096811099 DOI 10.1111/nph.17003 Anotace The parasitic genus Cuscuta (Convolvulaceae) is exceptional among plants with respect to centromere organization, including both monocentric and holocentric chromosomes, and substantial variation in genome size and chromosome number. We investigated 12 species representing the diversity of the genus in a phylogenetic context to reveal the molecular and evolutionary processes leading to the diversification of their genomes.
We measured genome sizes and investigated karyotypes and centromere organization using molecular cytogenetic techniques. We also performed low-pass whole genome sequencing and comparative analysis of repetitive DNA composition.
A remarkable 102-fold variation in genome sizes (342-34 734 Mbp/1C) was detected for monocentric Cuscuta species, while genomes of holocentric species were of moderate sizes (533-1545 Mbp/1C). The genome size variation was primarily driven by the differential accumulation of LTR-retrotransposons and satellite DNA. The transition to holocentric chromosomes in the subgenus Cuscuta was associated with loss of histone H2A phosphorylation and elimination of centromeric retrotransposons. In addition, basic chromosome number of holocentric species (x = 7) was smaller than in monocentric (x = 15 or 16).
We demonstrated that the transition to holocentricity in Cuscuta was accompanied by significant changes in epigenetic marks, chromosome number and the repetitive DNA sequence composition.Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17003
Počet záznamů: 1