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A selective autophagy cargo receptor NBR1 modulates abscisic acid signalling in Arabidopsis thaliana
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SYSNO ASEP 0532721 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název A selective autophagy cargo receptor NBR1 modulates abscisic acid signalling in Arabidopsis thaliana Tvůrce(i) Tarnowski, L. (PL)
Rodriguez, M. C. (PL)
Brzywczy, J. (PL)
Piecho-Kabacik, M. (PL)
Krčkova, Zuzana (UEB-Q) ORCID
Martinec, Jan (UEB-Q) RID, ORCID
Wawrzynska, A. (PL)
Sirko, A. (PL)Celkový počet autorů 8 Číslo článku 7778 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 10, č. 1 (2020)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova UBIQUITIN-PROTEASOME SYSTEM ; TOR KINASE ; PROTEIN Vědní obor RIV ED - Fyziologie Obor OECD Biochemistry and molecular biology CEP LTC17084 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000560353500003 EID SCOPUS 85084306930 DOI 10.1038/s41598-020-64765-z Anotace The plant selective autophagy cargo receptor neighbour of breast cancer 1 gene (NBR1) has been scarcely studied in the context of abiotic stress. We wanted to expand this knowledge by using Arabidopsis thaliana lines with constitutive ectopic overexpression of the AtNBR1 gene (OX lines) and the AtNBR1 Knock-Out (KO lines). Transcriptomic analysis of the shoots and roots of one representative OX line indicated differences in gene expression relative to the parental (WT) line. In shoots, many differentially expressed genes, either up- or down-regulated, were involved in responses to stimuli and stress. In roots the most significant difference was observed in a set of downregulated genes that is mainly related to translation and formation of ribonucleoprotein complexes. The link between AtNBR1 overexpression and abscisic acid (ABA) signalling was suggested by an interaction network analysis of these differentially expressed genes. Most hubs of this network were associated with ABA signalling. Although transcriptomic analysis suggested enhancement of ABA responses, ABA levels were unchanged in the OX shoots. Moreover, some of the phenotypes of the OX (delayed germination, increased number of closed stomata) and the KO lines (increased number of lateral root initiation sites) indicate that AtNBR1 is essential for fine-tuning of the ABA signalling pathway. The interaction of AtNBR1 with three regulatory proteins of ABA pathway (ABI3, ABI4 and ABI5) was observed in planta. It suggests that AtNBR1 might play role in maintaining the balance of ABA signalling by controlling their level and/or activity. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2021 Elektronická adresa http://doi.org/10.1038/s41598-020-64765-z
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