Počet záznamů: 1  

Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry

  1. 1.
    SYSNO ASEP0531800
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevAnalysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry
    Tvůrce(i) Ďurč, P. (CZ)
    Dosedělová, V. (CZ)
    Foret, František (UIACH-O) RID, ORCID
    Dolina, J. (CZ)
    Konečný, Š. (CZ)
    Himmelsbach, M. (AT)
    Buchberger, W. (AT)
    Kubáň, Petr (UIACH-O) ORCID
    Celkový počet autorů8
    Číslo článku461278
    Zdroj.dok.Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
    Roč. 1625, AUG (2020), s. 1-9
    Poč.str.9 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovaBile acids ; hplc-ms ; Saliva
    Vědní obor RIVCB - Analytická chemie, separace
    Obor OECDAnalytical chemistry
    Způsob publikováníOmezený přístup
    Institucionální podporaUIACH-O - RVO:68081715
    UT WOS000552017600024
    EID SCOPUS85085840766
    DOI10.1016/j.chroma.2020.461278
    AnotaceA fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease. (C) 2020 Elsevier B.V. All rights reserved.
    PracovištěÚstav analytické chemie
    KontaktIveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
    Rok sběru2021
    Elektronická adresahttp://hdl.handle.net/11104/0310416
Počet záznamů: 1  

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