Počet záznamů: 1
Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry
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SYSNO ASEP 0531800 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry Tvůrce(i) Ďurč, P. (CZ)
Dosedělová, V. (CZ)
Foret, František (UIACH-O) RID, ORCID
Dolina, J. (CZ)
Konečný, Š. (CZ)
Himmelsbach, M. (AT)
Buchberger, W. (AT)
Kubáň, Petr (UIACH-O) ORCIDCelkový počet autorů 8 Číslo článku 461278 Zdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1625, AUG (2020), s. 1-9Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Bile acids ; hplc-ms ; Saliva Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry Způsob publikování Omezený přístup Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000552017600024 EID SCOPUS 85085840766 DOI 10.1016/j.chroma.2020.461278 Anotace A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease. (C) 2020 Elsevier B.V. All rights reserved. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2021 Elektronická adresa http://hdl.handle.net/11104/0310416
Počet záznamů: 1