Počet záznamů: 1
Antiviral Activity and Adaptive Evolution of Avian Tetherins
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SYSNO ASEP 0525557 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Antiviral Activity and Adaptive Evolution of Avian Tetherins Tvůrce(i) Krchlíková, Veronika (UMG-J)
Fabryova, H. (US)
Hron, Tomáš (UMG-J)
Young, J.M. (US)
Koslová, Anna (UMG-J)
Hejnar, Jiří (UMG-J) RID
Strebel, K. (US)
Elleder, Daniel (UMG-J) RIDCelkový počet autorů 8 Číslo článku e00416-20 Zdroj.dok. Journal of Virology - ISSN 0022-538X
Roč. 94, č. 12 (2020)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova avian retrovirus ; chicken ; restriction factor ; tetherin Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Virology CEP GA17-23675S GA ČR - Grantová agentura ČR LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UMG-J - RVO:68378050 UT WOS 000537852600016 DOI 10.1128/JVI.00416-20 Anotace Tetherin/BST-2 is an antiviral protein that blocks the release of enveloped viral particles by linking them to the membrane of producing cells. At first, BST-2 genes were described only in humans and other mammals. Recent work identified BST-2 orthologs in nonmammalian vertebrates, including birds. Here, we identify the BST-2 sequence in domestic chicken (Gallus gallus) for the first time and demonstrate its activity against avian sarcoma and leukosis virus (ASLV). We generated a BST-2 knockout in chicken cells and showed that BST-2 is a major determinant of an interferon-induced block of ASLV release. Ectopic expression of chicken BST-2 blocks the release of ASLV in chicken cells and of human immunodeficiency virus type 1 (HIV-1) in human cells. Using metabolic labeling and pulse-chase analysis of HIV-1 Gag proteins, we verified that chicken BST-2 blocks the virus at the release stage. Furthermore, we describe BST-2 orthologs in multiple avian species from 12 avian orders. Previously, some of these species were reported to lack BST-2, highlighting the difficulty of identifying sequences of this extremely variable gene. We analyzed BST-2 genes in the avian orders Galliformes and Passeriformes and showed that they evolve under positive selection. This indicates that avian BST-2 is involved in host-virus evolutionary arms races and suggests that BST-2 antagonists exist in some avian viruses. In summary, we show that chicken BST-2 has the potential to act as a restriction factor against ASLV. Characterizing the interaction of avian BST-2 with avian viruses is important in understanding innate antiviral defenses in birds. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2021 Elektronická adresa https://jvi.asm.org/content/94/12/e00416-20
Počet záznamů: 1