Počet záznamů: 1
Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex
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SYSNO ASEP 0518368 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Electrodeposition of Silver Amalgam on Thin Gold Film Electrodes for Voltammetric Detection of 4-Nitrophenol and DNA Labeled with Osmium Tetroxide-Bipyridine Complex Tvůrce(i) Havranová-Vidláková, Pavlína (BFU-R) ORCID
Ligmajer, Filip (BFU-R) ORCID
Daňhel, Aleš (BFU-R) RID, ORCIDCelkový počet autorů 3 Zdroj.dok. Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 31, č. 10 (2019), s. 1952-1960Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova nucleic-acid ; carbon ; nanoparticles ; electrochemistry Vědní obor RIV CG - Elektrochemie Obor OECD Analytical chemistry CEP GJ17-23634Y GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000474183900001 DOI 10.1002/elan.201900306 Anotace Alternative electrode materials suitable to prepare novel working electrode applicable in detecting biopolymers such as nucleic acids, proteins or glycoproteins, represent a significant contribution to bio-electroanalysis. Herein, electrodes made of vapor-deposited thin gold films (vAuE) were used as an alternative substrate for the electrodeposition of silver amalgam particles (AgAPs), next to indium tin oxide and pyrolytic graphite, which are already used. The conditions and parameters of double pulse chronoamperometry were optimized for the most-sensitive voltammetric detection of 4-nitrophenol (4-NP). The resulting electrodes were characterized by scanning electron microscope with energy dispersive X-ray spectroscopy. While 4-NP could not be detected by bare nonactivated vAuEs at all, their electrochemical activation offered a limit of detection (LoD) of 25 and 5 mu mol.l(-1) by means of CV and DPV, respectively. AgAP electrodeposited on vAuE, offered 2.5-times lower LoDs 10 mu mol.l(-1) by CV and comparable LoD 5 mu mol.l(-1) by DPV. Advantageously, AgAPs could be repeatedly deposited on and anodically dissolved from the vAuE with a relative standard deviation 13 % of the ten-times repeated DPV signal of 4-NP (100 mu mol.l(-1)). In comparison to vAuE, the vAuE-AgAP offered about 400 mV broader potential window, which allowed detection of single strand DNA fragment labeled by osmium tetroxide-bipyridine complex down to 2 ng.mu l(-1) by means of DPV. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2020 Elektronická adresa http://dspace.lib.vutbr.cz/xmlui/bitstream/11012/184643/1/2019%20Electroanalsis%20Havranova%20Preprint.pdf
Počet záznamů: 1