Počet záznamů: 1
Combined photometric detector utilizing light emitting diodes, 50 nL silica capillary cell, and CCD spectrometer
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SYSNO ASEP 0509158 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Combined photometric detector utilizing light emitting diodes, 50 nL silica capillary cell, and CCD spectrometer Tvůrce(i) Šesták, Jozef (UIACH-O) ORCID
Kahle, Vladislav (UIACH-O) RIDCelkový počet autorů 2 Zdroj.dok. CECE 2019. - Gdańsk : University of Gdańsk, 2019
S. 45-45Poč.str. 1 s. Forma vydání Nosič - C Akce CECE 2019. International Interdisciplinary Meeting on Bioanalysis /16./ Datum konání 24.09.2019 - 26.09.2019 Místo konání Gdańsk Země PL - Polsko Typ akce WRD Jazyk dok. eng - angličtina Země vyd. PL - Polsko Klíč. slova capillary LC ; absorbance-fluorescence detector ; capillary detection cell ; CCD spectrometer ; light-emitting diodes Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP VI20172020069 GA MV - Ministerstvo vnitra Institucionální podpora UIACH-O - RVO:68081715 Anotace Many LC methods utilize absorbance and fluorescence detectors connected in series as a versatile detection strategy for quantitation of target fluorophores and non-fluorescent molecules within single run. However, the use of two bulky detectors is not a useful strategy for capillary LC (column I. D. typically less than 300 μm), where detector should be equipped with a miniaturised detection cell and positioned as close as possible to the column outlet to keep an extra column band broadening within acceptable limits. An effort to integrate absorbance and fluorescence monitoring into a single detection cell and detector device is thus a logical step. Here we present a compact photometric detector design which uses light emitting diodes (LEDs) as the light source, customized 50 nL L-shaped silica capillary detection cell [1], and CCD spectrometer as the light detector. 265 nm LED is used as the light source for measurement of absorption while 365 nm and 470 nm LEDs are used for fluorophore excitation. Presented results demonstrate 1 mm effective optical path of the detection cell and extremely low level of stray light such that upper limit of dynamic range is more than 2.5 AU. Response of the detector is linear over three orders of magnitude of concentration. Detection capabilities are demonstrated by LODs of uracil, anthracene and fluorescein of 2×10-6 M, 1×10-8 M, and 1×10-9 respectively. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2020
Počet záznamů: 1