Optical imaging and localization of prospective scattering labels smaller than a single protein

0504562 - ÚFE 2020 RIV GB eng J - Článek v odborném periodiku
Holanová, Kristýna - Vala, Milan - Piliarik, Marek
Optical imaging and localization of prospective scattering labels smaller than a single protein.
Optics and Laser Technology. -, č. 109 (2019), s. 323-327. ISSN 0030-3992. E-ISSN 1879-2545
Grant CEP: GA MŠMT(CZ) LL1602
Institucionální podpora: RVO:67985882
Klíčová slova: iSCAT * Nanoparticle detection * Interference scattering microscopy
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 3.233, rok: 2019
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S0030399218310818?via%3Dihub

Labeling of biological matter is a key technique enabling visualization and real-time tracking of dynamic processes beyond the diffraction limit. While the precision of fluorescence-based localization microscopy is limited by signal saturation, a new generation of small scattering labels (such as gold nanoparticles) have brought the localization precision below the size of a single protein. However, the size of scattering labels is also the most obvious handicap, as they are usually significantly larger than the resulting localization precision. We address this inconsistency and demonstrate detection and precise localization of gold nanoparticles of only 2 nm in diameter using interferometric detection of scattering (iSCAT). This size is considerably smaller than the size of a small protein and in combination with the possibility of tracking the nanoparticle position, we foresee a new concept of detailed studies of biomolecular systems in the future
Trvalý link: http://hdl.handle.net/11104/0296168