Počet záznamů: 1
H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions
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SYSNO ASEP 0501862 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions Tvůrce(i) Kovaříková, Alena (BFU-R)
Legartová, Soňa (BFU-R) ORCID
Krejčí, Jana (BFU-R) RID, ORCID
Bártová, Eva (BFU-R) ORCIDCelkový počet autorů 4 Zdroj.dok. Aging. - : Impact Journals LLC - ISSN 1945-4589
Roč. 10, č. 10 (2018), s. 2585-2605Poč.str. 21 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova double-strand breaks ; histone H3 ; cell-cycle ; heterochromatin protein-1 Vědní obor RIV BO - Biofyzika Obor OECD Cell biology CEP GA18-07384S GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000451851600013 DOI 10.18632/aging.101572 Anotace Methylation of histones H4 at lysine 20 position (H4K20me), which is functional in DNA repair, represents a binding site for the 53BP1 protein. Here, we show a radiation-induced increase in the level of H4K20me3 while the levels of H4K20me1 and H4K20me2 remained intact. H4K20me3 was significantly pronounced at DNA lesions in only the G1 phase of the cycle, while this histone mark was reduced in very late S and G2 phases when PCNA was recruited to locally micro-irradiated chromatin. H4K20me3 was diminished in locally irradiated Suv39h1/h2 double knockout (dn) fibroblasts, and the same phenomenon was observed for H3K9me3 and its binding partner, the HP1 beta protein. Immunoprecipitation showed the existence of an interaction between H3K9me3-53BP1 and H4K20me3-53BP1, however, HP1 beta did not interact with 53BP1. Together, H3K9me3 and H4K20me3 represent epigenetic markers that are important for the function of the 53BP1 protein in non-homologous end joining (NHEJ) repair. The very late S phase represents the cell cycle breakpoint when a DDR function of the H4K20me3-53BP1 complex is abrogated due to recruitment of the PCNA protein and other DNA repair factors of homologous recombination to DNA lesions. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2019
Počet záznamů: 1