Počet záznamů: 1
Nanostructures in Biomedicine: Their Regenerative and toxic properties
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SYSNO ASEP 0499259 Druh ASEP M - Kapitola v monografii Zařazení RIV C - Kapitola v knize Název Cytotoxicity of Nanomaterials Applicable in Restoration and Conservation Tvůrce(i) Brzicová, Táňa (UEM-P)
Vrbová, Kristýna (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Rössner ml., Pavel (UEM-P) RID, ORCIDZdroj.dok. Nanostructures in Biomedicine: Their Regenerative and toxic properties. - Praha 3 : Proxima Tisk, s.r.o., 2018 / Pamula Elzbieta - ISBN 978-80-906655-7-6 Rozsah stran s. 21-28 Poč.str. 8 s. Poč.str.knihy 198 Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova nanotechnology ; cytotoxicity ; restoration Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology CEP LO1508 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UEM-P - RVO:68378041 Anotace Nanotechnologies represent new promising approach for conservation and preservation of cultural heritage. Nanomaterialshave been shown to improve efficacy, durability and quality of the restoration due to their unique properties that can be achieved and tuned by controlling particle physico-chemical characteristics. The same characteristics, however, affect the way how nanomaterials interact with biological systems and may potentially underlie their enhanced or unexpected toxicity. In this pilot study, we compared the toxic potential of representatives of three of the most common oxide materials applicable in
restoration: TiO2 (standard and purified P25, a mixture of prevailing anatase with rutile crystalline modifications), SiO2 (bare A200, and R805, R9200 as coated forms of A200), and ZnO. Using two in vitro cytotoxicity assays, WST-1 and LDH, evaluating metabolic activity and cell membrane integrity, respectively, we preliminary ranked the tested substances according to their cytotoxic potential, which may be used for their prioritization for further testing and applications. After 24 h exposure, a dose-dependent decrease in cell viability was only detected in ZnO nanoparticles and uncoated silica A200). Hydrophobic coated silicas (R805 and R9200) and TiO2 nanoparticles (purified and unpurified P25) did not exhibit cytotoxic effects up to the highest tested concentration of 250 μg/mL. The tested nanomaterials were ranked according to their cytotoxic potential expressed as IC50. Toxicological data related to the physico-chemical characteristics will be applicable in developing both more efficient and safer nano-based products for restoration and conservation.
Pracoviště Ústav experimentální medicíny Kontakt Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Rok sběru 2019
Počet záznamů: 1