Počet záznamů: 1
Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics
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SYSNO ASEP 0495307 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dynamics of the Pollen Sequestrome Defined by Subcellular Coupled Omics Tvůrce(i) Hafidh, Said (UEB-Q) RID, ORCID
Potěšil, D. (CZ)
Müller, Karel (UEB-Q) RID, ORCID
Fíla, Jan (UEB-Q) RID, ORCID
Michailidis, Christos (UEB-Q) ORCID
Herrmannová, Anna (MBU-M) RID, ORCID
Feciková, Jana (UEB-Q) ORCID
Ischebeck, T. (DE)
Valášek, Leoš Shivaya (MBU-M) RID, ORCID
Zdráhal, Z. (CZ)
Honys, David (UEB-Q) RID, ORCIDCelkový počet autorů 11 Zdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 178, č. 1 (2018), s. 258-282Poč.str. 25 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova differentially expressed proteins ; male gametophyte development ; messenger-rna transport ; tube growth ; nicotiana-tabacum ; gene-expression ; arabidopsis-thaliana ; mature pollen ; proteomic analysis ; processing bodies Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP LQ1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015043 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-06238S GA ČR - Grantová agentura ČR GA18-02448S GA ČR - Grantová agentura ČR GA17-23183S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 ; MBU-M - RVO:61388971 UT WOS 000444004100022 EID SCOPUS 85054564041 DOI 10.1104/pp.18.00648 Anotace Reproduction success in angiosperm plants depends on robust pollen tube growth through the female pistil tissues to ensure successful fertilization. Accordingly, there is an apparent evolutionary trend to accumulate significant reserves during pollen maturation, including a population of stored mRNAs, that are utilized later for a massive translation of various proteins in growing pollen tubes. Here, we performed a thorough transcriptomic and proteomic analysis of stored and translated transcripts in three subcellular compartments of tobacco (Nicotiana tabacum), long-term storage EDTA/puromycin-resistant particles, translating polysomes, and free ribonuclear particles, throughout tobacco pollen development and in in vitro-growing pollen tubes. We demonstrated that the composition of the aforementioned complexes is not rigid and that numerous transcripts were redistributed among these complexes during pollen development, which may represent an important mechanism of translational regulation. Therefore, we defined the pollen sequestrome as a distinct and highly dynamic compartment for the storage of stable, translationally repressed transcripts and demonstrated its dynamics. We propose that EDTA/puromycin-resistant particle complexes represent aggregated nontranslating monosomes as the primary mediators of messenger RNA sequestration. Such organization is extremely useful in fast tip-growing pollen tubes, where rapid and orchestrated protein synthesis must take place in specific regions. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2019
Počet záznamů: 1