Počet záznamů: 1
Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells
- 1.
SYSNO ASEP 0486725 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells Tvůrce(i) Ohradanova-Repic, A. (AT)
Nogueira, E. (PT)
Hartl, I. (AT)
Gomes, A.C. (PT)
Preto, A. (PT)
Steinhuber, E. (AT)
Muehlgrabner, V. (AT)
Repic, M. (AT)
Kuttke, M. (AT)
Zwirzitz, A. (AT)
Prouza, M. (CZ)
Suchánek, M. (CZ)
Wozniak-Knopp, G. (AT)
Hořejší, Václav (UMG-J) RID
Schabbauer, G. (AT)
Cavaco-Paulo, A. (AT)
Stockinger, H. (AT)Celkový počet autorů 17 Zdroj.dok. Nanomedicine: Nanotechnology, Biology and Medicine. - : Elsevier - ISSN 1549-9634
Roč. 14, č. 1 (2018), s. 123-130Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Active targeting ; Liposome functionalization ; Immunoliposome ; Antibody engineering ; Recombinant Fab antibody fragment Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology Institucionální podpora UMG-J - RVO:68378050 UT WOS 000423842300012 DOI 10.1016/j.nano.2017.09.003 Anotace Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers. (C) 2017 Elsevier Inc. All rights reserved. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2018
Počet záznamů: 1