Počet záznamů: 1
Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters
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SYSNO ASEP 0483264 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters Tvůrce(i) Zhang, T. (US)
Galdieri, L. (US)
Hašek, Jiří (MBU-M) RID, ORCID
Vančura, A. (US)Číslo článku fnx227 Zdroj.dok. FEMS Microbiology Letters. - : Oxford University Press - ISSN 0378-1097
Roč. 364, č. 22 (2017)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova phospholipase C ; casein kinase I ; hexose transporters Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA16-05497S GA ČR - Grantová agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000416731600010 DOI 10.1093/femsle/fnx227 Anotace Phospholipase C (Plc1p) in Saccharomyces cerevisiae is required for normal degradation of repressor Mth1p and expression of the HXT genes encoding cell membrane transporters of glucose. Plc1p is also required for normal localization of glucose transporters to the cell membrane. Consequently, plc1 Delta cells display histone hypoacetylation and transcriptional defects due to reduced uptake and metabolism of glucose to acetyl-CoA, a substrate for histone acetyltransferases. In the presence of glucose, Mth1p is phosphorylated by casein kinase I Yck1/2p, ubiquitinated by the SCFGrr1 complex and degraded by the proteasome. Here, we show that while Plc1p does not affect the function of the SCFGrr1 complex or the proteasome, it is required for normal protein level of Yck2p. Since stability of Yck1/2p is regulated by a glucose-dependent mechanism, PLC1 inactivation results in destabilization of Yck1/2p and defect in Mth1p degradation. Based on our results and published data, we propose a model in which plc1 Delta mutation causes increased internalization of glucose transporters, decreased transport of glucose into the cells, and consequently decreased stability of Yck1/2p, increased stability of Mth1p and decreased expression of the HXT genes. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2018
Počet záznamů: 1