Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy

0477926 - ÚPT 2018 RIV GB eng J - Článek v odborném periodiku
Rebrošová, K. - Šiler, Martin - Samek, Ota - Růžička, F. - Bernatová, Silvie - Ježek, Jan - Zemánek, Pavel - Holá, V.
Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy.
Future Microbiology. Roč. 12, č. 10 (2017), s. 881-890. ISSN 1746-0913. E-ISSN 1746-0921
Grant CEP: GA ČR(CZ) GA15-20645S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: Raman spectroscopy * rapid diagnostics * Staphylococcus epidermidis * Staphyococcus aureus
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 3.190, rok: 2017

Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material Staphylococcus aureus and Staphylococcus epidermidis using this rapid noninvasive technique. Materials & methods: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. Discussion & conclusion: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
Trvalý link: http://hdl.handle.net/11104/0274138