Počet záznamů: 1
Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography
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SYSNO ASEP 0469740 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Bridged polysilsesquioxane-based wide-bore monolithic capillary columns for hydrophilic interaction chromatography Tvůrce(i) Šesták, Jozef (UIACH-O) ORCID
Moravcová, Dana (UIACH-O) RID, ORCID
Křenková, Jana (UIACH-O) RID, ORCID
Planeta, Josef (UIACH-O) RID, ORCID
Roth, Michal (UIACH-O) RID, ORCID
Foret, František (UIACH-O) RID, ORCIDCelkový počet autorů 6 Zdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1479, JAN (2017), s. 204-209Poč.str. 6 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova silica monolithic column ; HILIC ; oligosaccharides ; nucleosides Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GA14-06319S GA ČR - Grantová agentura ČR GA16-03749S GA ČR - Grantová agentura ČR Institucionální podpora UIACH-O - RVO:68081715 UT WOS 000392681300023 EID SCOPUS 85008244059 DOI 10.1016/j.chroma.2016.11.062 Anotace The synthesis and characterization of large-bore silica-based monolithic capillary columns (0.32 mm × 150 mm) are presented. Columns were prepared by acidic hydrolysis of a mixture containing tetramethoxysilane (TMOS) and 1,2-bis(trimethoxysilyl)ethane (BTME) in different molar ratios in the presence of polyethylene glycol and urea. The monoliths were modified by zwitterionic monomer [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide via 3-(trimethoxysilyl)propyl methacrylate. Prepared stationary phases were evaluated by scanning electron microscopy and chromatographic separation of nucleobases and their derivatives in the HILIC mode. The best chromatographic results were obtained with the column prepared from the reaction mixture containing BTME and TMOS in a 1:4 molar ratio. The permeability of such column reached 1.68 × 10−14 m2 and the efficiency, expressed as a height equivalent of the theoretical plate, did not exceed 10.5 μm for the tested compounds. The columns were successfully applied to HILIC separation of native and labeled oligosaccharides and glycans released from bovine ribonuclease B and human immunoglobulin G. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2017
Počet záznamů: 1