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Acute temperature effects on function of the chick embryonic heart
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SYSNO ASEP 0465930 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Acute temperature effects on function of the chick embryonic heart Tvůrce(i) Vostárek, František (FGU-C) RID, ORCID, SAI
Svatůňková, Jarmila (FGU-C)
Sedmera, David (FGU-C) RID, ORCID, SAIZdroj.dok. Acta Physiologica. - : Wiley - ISSN 1748-1708
Roč. 217, č. 4 (2016), s. 276-286Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova arrhythmias ; calcium imaging ; chick embryo ; conduction block ; heart development ; optical mapping Vědní obor RIV EA - Morfologické obory a cytologie CEP GAP302/11/1308 GA ČR - Grantová agentura ČR GA13-12412S GA ČR - Grantová agentura ČR GA16-02972S GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000379972300005 EID SCOPUS 84978933172 DOI 10.1111/apha.12691 Anotace We analysed the effects of acute temperature change on the beating rate, conduction properties and calcium transients in the chick embryonic heart invitro and in ovo. MethodsThe effects of temperature change (34, 37 and 40 degrees C) on calcium dynamics in isolated ED4 chick hearts invitro were investigated by high-speed calcium optical imaging. For comparison and validation of invitro measurements, experiments were also performed in ovo using videomicroscopy. Artificial stimulation experiments were performed invitro and inovo to uncover conduction limits of heart segments. ResultsDecrease in temperature from 37 to 34 degrees C invitro led to a 22% drop in heart rate and unchanged amplitude of Ca2+ transients, compared to a 25% heart rate decrease in ovo. Increase in temperature from 37 to 40 degrees C invitro and in ovo led to 20 and 23% increases in heart rate, respectively, and a significant decrease in amplitude of Ca2+ transients (atrium -35%, ventricle -38%). We observed a wide spectrum of arrhythmias invitro, of which the most common was atrioventricular (AV) block (57%). There was variability of AV block locations. Pacing experiments invitro and in ovo suggested that the AV blocks were likely caused by relative tissue hypoxia and not by the tachycardia itself. ConclusionThe pacemaker and AV canal are the most temperature-sensitive segments of the embryonic heart. We suggest that the critical point for conduction is the connection of the ventricular trabecular network to the AV canal. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
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