Počet záznamů: 1
Sperm membrane protein dynamics during the acrosome reaction
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SYSNO ASEP 0463080 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV Záznam nebyl označen do RIV Název Sperm membrane protein dynamics during the acrosome reaction Tvůrce(i) Děd, Lukáš (BTO-N) RID
Frolíková, Michaela (BTO-N) ORCID
Šebková, Nataša (BTO-N)
Kubátová, Alena (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)Celkový počet autorů 5 Zdroj.dok. Book of abstracts of the 49th Annual meeting - Systems biology of Reproduction. - Madison : Society for the Study of Reproduction, 2016 / Murphy Brian Rozsah stran s. 181-181 Poč.str. 1 s. Akce 49th Annual meeting - Systems biology of Reproduction Datum konání 16.07.2016-20.07.2016 Místo konání San Diego Země US - Spojené státy americké Typ akce WRD Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova protein dynamics ; sperm capacitation ; acrosome reaction ; CD46 ; Duolink assay ; ezrin/radixin/moezin Vědní obor RIV EC - Imunologie CEP ED 1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA 14-05547S GA ČR - Grantová agentura ČR Institucionální podpora BTO-N - RVO:86652036 Anotace Sperm membrane protein relocation is a key process in human and mammalian reproduction. During sperm acrosome reaction, some key molecules responsible for binding and fusion with the egg plasma membrane have to be relocated to the sperm surface and even more specifically to the equatorial segment, where fusion process takes a place. In previous studies, the unique presence of complement regulatory protein CD46 in sperm acrosomal membrane was shown. CD46 protein has been shown to play an important role in the acrosomal stability and its proper expression and protein abundance in the acrosomal membrane is important for the balanced ability of individual sperm cell and whole sperm population to undergo precisely timed acrosome reaction. However, the mechanism of CD46 action in sperm and its connection with other sperm proteins has not been explored further. Here, using fluorescence microscopy we demonstrate the specific localization and relocation pattern of the CD46 molecule during capacitation and acrosome reaction. Furthermore, using Duolink assay we uncovered the specific interaction of CD46 protein molecules with other specific membrane proteins, which belong to the integrin protein superfamily. Finally, the specific topological patterns of CD46 and integrin protein clusters within the acrosomal cap, equatorial and post-acrosomal regions of the sperm head have been examined by STED super-resolution microscopy. Our results showed, that CD46 and integrins specifically interact during the process of acrosome reaction and their interaction is vital for their joint relocation. This relocation then leads to the establishing of specific membrane protein content in the equatorial region of the sperm head, which later interact with its counterpart on the egg plasma membrane and thus facilitate sperm-egg fusion. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1