Počet záznamů: 1
Cellular responses modulated by FGF-2 adsorbed on albumin/heparin layer-by-layer assemblies
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SYSNO ASEP 0444496 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Cellular responses modulated by FGF-2 adsorbed on albumin/heparin layer-by-layer assemblies Tvůrce(i) Kumorek, Marta M. (UMCH-V) RID
Kubies, Dana (UMCH-V) RID, ORCID
Filová, Elena (FGU-C) RID, ORCID
Houska, Milan (UMCH-V) RID
Kasoju, Naresh (UMCH-V) RID, ORCID
Mázl Chánová, Eliška (UMCH-V) RID
Matějka, Roman (FGU-C) RID, ORCID, SAI
Krýslová, Markéta (FGU-C)
Bačáková, Lucie (FGU-C) RID, ORCID
Rypáček, František (UMCH-V) RIDZdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 10, č. 5 (2015), e0125484_1-e0125484_23Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova self-assembly ; layer-by-layer ; heparin Vědní obor RIV CD - Makromolekulární chemie Vědní obor RIV – spolupráce Fyziologický ústav - Biotechnologie a bionika CEP EE2.3.30.0029 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GAP108/11/1857 GA ČR - Grantová agentura ČR GPP108/12/P624 GA ČR - Grantová agentura ČR ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UMCH-V - RVO:61389013 ; FGU-C - RVO:67985823 UT WOS 000354049700072 EID SCOPUS 84929191214 DOI 10.1371/journal.pone.0125484 Anotace In a typical cell culture system, growth factors immobilized on the cell culture surfaces can serve as a reservoir of bio-signaling molecules, without the need to supplement them additionally into the culture medium. In this paper, we report on the fabrication of albumin/heparin (Alb/Hep) assemblies for controlled binding of basic fibroblast growth factor (FGF-2). The surfaces were constructed by layer-by-layer adsorption of polyelectrolytes albumin and heparin and were subsequently stabilized by covalent crosslinking with glutaraldehyde. An analysis of the surface morphology by atomic force microscopy showed that two Alb/Hep bilayers are required to cover the surface of substrate. The formation of the Alb/Hep assemblies was monitored by the surface plasmon resonance (SPR), the infrared multiinternal reflection spectroscopy (FTIR MIRS) and UV/VIS spectroscopy. The adsorption of FGF-2 on the cross-linked Alb/Hep was followed by SPR. The results revealed that FGF-2 binds to the Alb/Hep assembly in a dose and time-dependent manner up to the surface concentration of 120 ng/cm2. The bioactivity of the adsorbed FGF-2 was assessed in experiments in vitro, using calf pulmonary arterial endothelial cells (CPAE). CPAE cells could attach and proliferate on Alb/Hep surfaces. The adsorbed FGF-2 was bioactive and stimulated both the proliferation and the differentiation of CPAE cells. The improvement was more pronounced at a lower FGF-2 surface concentration (30 ng/cm2) than on surfaces with a higher concentration of FGF-2 (120 ng/cm2). Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2016
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