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One more drop for decreasing reproduction
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SYSNO ASEP 0428374 Druh ASEP K - Konferenční příspěvek (lokální konf.) Zařazení RIV Záznam nebyl označen do RIV Název One more drop for decreasing reproduction Tvůrce(i) Dvořáková-Hortová, K. (CZ)
Šídlová, A. (CZ)
Děd, Lukáš (BTO-N) RID
Hladovcová, D. (CZ)
Vieweg, M. (DE)
Weidner, W. (DE)
Steger, K. (DE)
Stopka, P. (CZ)
Paradowska-Dogan, A. (DE)Celkový počet autorů 9 Zdroj.dok. Book of abstracts XXth Symposium of Biology and Immunology of Reproduction with international participation. - Praha : Biotechnologický ústav AVČR, v.v.i, 2014
S. 1-82Poč.str. 2 s. Akce XXth Symposium of Biology and Immunology of Reproduction with international participation Datum konání 22.05.2014-24.05.2014 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Toxoplasma gondii ; luteinizing hormone ; spermatocytes ; methylation Vědní obor RIV EE - Mikrobiologie, virologie CEP 14-055547S GA ČR - Grantová agentura ČR Anotace Toxoplasma gondii is a common protozoan parasite that infects warm-blooded animals throughout the world, including mice and humans. During infection, both, the parasite and the host, utilize various mechanisms to maximize their own reproductive success. Mice and humans are both the intermediate hosts for Toxoplasma gondii, which forms specialized vacuoles containing reproductive cysts in the formers’ tissue. As half of the human population is infected, developing a disease called toxoplasmosis, along with an ever-growing number of couples suffering with idiopathic infertility, it is therefore surprising that there is a lack of research on how T.gondii can alter reproductive parameters. In this study, a detailed histometric screening of the testicular function along with the levels of the pituitary luteinizing hormone (LH) were analysed in infected mice. Data on relative testis and epididymis weight, and sperm count were also collected. Based on the results obtained, the level of LH in the urine of Toxoplasma gondii infected mice was lower compared to the control. In direct correlation with the hormone level, testicular function and sperm production was also significantly lower in T. gondii positive group using sperm count and histometric analysis as a marker. Not only were the number of leptotene primary spermatocytes and spermatids lowered, but the number of Sertoli cells and the tubule diameter were elevated. In parallel, a pilot epigenetic study on global testicular methylation, and specific methylation of Crem, Creb1 and Hspa1genes essential for successfully ongoing spermatogenesis was performed. Global methylation was elevated in Toxoplasma infected mice, and differences in the DNA methylation of selected genes were detected between the Toxoplasma positive and control group. These findings demonstrate a direct relation between T. gondii infection and the decrease of male reproductive fitness in mice, which may contribute to an increase of infertility in humans. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2015
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