Počet záznamů: 1
De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies
- 1.0421247 - BC 2014 RIV US eng J - Článek v odborném periodiku
Schwarz, Alexandra - von Reumont, B.M. - Erhart, Jan - Chagas, A. C. - Ribeiro, J.M.C. - Kotsyfakis, Michalis
De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies.
FASEB Journal. Roč. 27, č. 12 (2013), s. 4745-4756. ISSN 0892-6638. E-ISSN 1530-6860
Grant CEP: GA ČR GAP502/12/2409
Institucionální podpora: RVO:60077344
Klíčová slova: cDNA * high-throughput annotation * molecular evolution * public database * tick feeding * tick life stages
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.480, rok: 2013
Tick salivary gland (SG) proteins possess powerful pharmacologic properties that facilitate tick feeding and pathogen transmission. For the first time, SG transcriptomes of Ixodes ricinus, an important disease vector for humans and animals, were analyzed using next-generation sequencing. SGs were collected from different tick life stages fed on various animal species, including cofeeding of nymphs and adults on the same host. Four cDNA samples were sequenced, discriminating tick SG transcriptomes of early- and late-feeding nymphs or adults. In total, 441,381,454 pyrosequencing reads and 67,703,183 Illumina reads were assembled into 272,220 contigs, of which 34,560 extensively annotated coding sequences are disclosed; 8686 coding sequences were submitted to GenBank. Overall, 13% of contigs were classified as secreted proteins that showed significant differences in the transcript representation among the 4 SG samples, including high numbers of sample-specific transcripts. Detailed phylogenetic reconstructions of two relatively abundant SG-secreted protein families demonstrated how this study improves our understanding of the molecular evolution of hematophagy in arthropods. Our data significantly increase the available genomic information for I. ricinus and form a solid basis for future tick genome/transcriptome assemblies and the functional analysis of effectors that mediate the feeding physiology and parasite-vector interaction of I. ricinus.
Trvalý link: http://hdl.handle.net/11104/0227694
Počet záznamů: 1