Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli
1.
SYSNO ASEP
0370645
Druh ASEP
C - Konferenční příspěvek (mezinárodní konf.)
Zařazení RIV
Záznam nebyl označen do RIV
Název
Expression of osmotin, an antifungal protein from Nicotiana tabacum in Escherichia coli
Tvůrce(i)
Viktorová, J. (CZ) Macková, M. (CZ) Macek, Tomáš (UOCHB-X)
Celkový počet autorů
3
Zdroj.dok.
Biologically Active Peptides. 12th Conference. - Praha : Institute of Organic Chemistry and Biochemistry AS CR, v. v. i, 2011 / Slaninová J.
- ISBN 978-80-86241-44-9
Rozsah stran
s. 154-155
Poč.str.
2 s.
Akce
Biologically Active Peptides /12./
Datum konání
27.04.2011-29.04.2011
Místo konání
Praha
Země
CZ - Česká republika
Typ akce
EUR
Jazyk dok.
eng - angličtina
Země vyd.
CZ - Česká republika
Klíč. slova
pathogenesis-related proteins ; osmotin ; antifungal activity ; recombinant protein
Vědní obor RIV
CC - Organická chemie
CEZ
AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS
000393738100041
Anotace
Plants have evolved a huge variety of proteins involved in the defense against pathogens and adaptation to stressful environments. Plant proteins whose expression is strongly induced in response to infection by pathogens belong to the group of pathogenesis-related (PR) proteins. The family of PR-5 proteins constitutes a group of cysteine-rich proteins including thaumatin, zeamatin and also osmotin. Osmotin is a cationic protein of 205 residues and molecular weight of 24 kDa. It was discovered and characterized in cells of Nicotiana tabacum var. Wisconsin 38. The plasmid harbouring cDNA of osmotin from Nicotiana tabacum was constructed for transformation of Escherichia coli. The osmotin gene was prepared in fusion with histidine tail to facilitate the isolation and purification from bacterial cells. Selection of transgenic colonies was based on antibiotic resistance. The hexahistidine-tagged osmotin was overexpressed in heterologous system by using pET expression vector and purified using immobilized metal affinity chromatography. The expression of osmotin was detected and antifungal activity was tested.
