Počet záznamů: 1
Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin
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SYSNO ASEP 0330518 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Oligomerization is involved in pore formation by Bordetella adenylate cyclase toxin Tvůrce(i) Vojtová, Jana (MBU-M) RID, ORCID
Basler, Marek (MBU-M)
Osička, Radim (MBU-M) RID, ORCID
Knapp, O. (DE)
Maier, E. (DE)
Černý, J. (CZ)
Benada, Oldřich (MBU-M) ORCID, RID
Benz, R. (DE)
Šebo, Peter (MBU-M) RID, ORCIDZdroj.dok. FASEB Journal. - : Wiley - ISSN 0892-6638
Roč. 23, - (2009), s. 2831-2843Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova blue native electrophoresis ; planar lipid bilayer membranes ; pore-forming activity Vědní obor RIV EE - Mikrobiologie, virologie CEP IAA500200914 GA AV ČR - Akademie věd 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000270241000006 DOI 10.1096/fj.09-131250 Anotace The Bordetella adenylate cyclase-hemolysin (CyaA, ACT, or AC-Hly) is a multifunctional toxin. CyaA delivers into host cells an adenylate cyclase enzyme (AC) and permeabilizes cell membrane by forming small cation selective pores. Indirect evidence suggested that these two activities were accomplished by different membrane-inserted CyaA conformers, one acting as an AC-delivering monomer, the other as an uncharacterized pore-forming oligomer. Here, CyaA oligomers were revealed in sheep erythrocyte membranes by immunogold labeling and directly demonstrated by pull-down of membrane-inserted CyaA together with biotinylated CyaA-AC- toxoid. Membrane oligomers of CyaA could also be resolved by non-denaturing electrophoresis of mild detergent extracts of erythrocytes Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2010
Počet záznamů: 1