Počet záznamů: 1
Comparative Analysis of the Arabidopsis Pollen Transcriptome
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SYSNO ASEP 0172314 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Comparative Analysis of the Arabidopsis Pollen Transcriptome Tvůrce(i) Honys, David (UEB-Q) RID, ORCID
Twell, D. (GB)Zdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 132, - (2003), s. 640ů652Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova transcriptome profiling ; Arabidopsis pollen ; male gametophyte Vědní obor RIV EB - Genetika a molekulární biologie CEP IAA5038207 GA AV ČR - Akademie věd CEZ AV0Z5038910 - UEB-Q MSM 113100003 Anotace We present a genome-wide view of the male gametophytic transcriptome in Arabidopsis based on microarray analysis. In comparison with the transcriptome of the sporophyte throughout development, the pollen transcriptome showed reduced complexity and a unique composition. We identified 992 pollen-expressed mRNAs, nearly 40% of which were detected specifically in pollen. Analysis of the functional composition of the pollen transcriptome revealed the over-representation of mRNAs encoding proteins involved in cell wall metabolism, cytoskeleton, and signaling and under-representation of mRNAs involved in transcription and protein synthesis. For several gene families, we observed a common pattern of mutually exclusive gene expression between pollen and sporophytic tissues for different gene family members. Our results provide a 50-fold increase in the knowledge of genes expressed in Arabidopsis pollen. Moreover, we also detail the extensive overlap (61%) of the pollen transcriptome with that of the sporophyte, wich provides ample potential to influence sporophytic fitness through gametophytic selection. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2004
Počet záznamů: 1