Molecular cloning of the prothoracicotropic hormone from the tobacco hornworm, Manduca sexta
1.
SYSNO ASEP
0157661
Druh ASEP
J - Článek v odborném periodiku
Zařazení RIV
J - Článek v odborném periodiku
Poddruh J
Ostatní články
Název
Molecular cloning of the prothoracicotropic hormone from the tobacco hornworm, Manduca sexta
Tvůrce(i)
Shionoya, M. (JP) Matsubayashi, H. (JP) Asahina, Masako (PAU-O) Kuniyoshi, H. (JP) Nagata, S. (JP) Riddiford, L. M. (US) Kataoka, H. (JP)
Zdroj.dok.
Insect Biochemistry and Molecular Biology. - : Elsevier
- ISSN 0965-1748
Roč. 33, č. 8 (2003), s. 795-801
Poč.str.
7 s.
Jazyk dok.
eng - angličtina
Země vyd.
GB - Velká Británie
Klíč. slova
prothoracicotropic hormone ; PTTH ; Manduca sexta
Vědní obor RIV
EB - Genetika a molekulární biologie
CEZ
AV0Z6022909 - PAU-O, BC-A
Anotace
A cDNA encoding a putative precursor of prothoracicotropic hormone (PTTH) from the tobacco hornworm, Manduca sexta, was isolated and sequenced. This clone contains an open reading frame encoding a 226-amino acid prepropeptide hormone. The deduced amino acid sequence is composed of a signal sequence, a precursor domain and a mature hormone and shows similarities to the other PTTHs that have been cloned from closely related lepidopteran species, Bombyx mori, Samia cynthia ricini, Antheraea peryni, and Hyalophora cecropia. The recombinant putative Manduca PTTH produced in E. coli was biologically active, both causing a larval molt in neck-ligated Manduca 4th instar larvae (ED50=50 pM) and the adult molt of diapausing Manduca pupae (ED50=79 pM), but was unable to stimulate molting of debrained Bombyx pupae.
