Počet záznamů: 1  

From fish to cells: Establishment of continuous cell lines from embryos of annual killifish Nothobranchius furzeri and N. kadleci

  1. 1.
    SYSNO ASEP0571795
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevFrom fish to cells: Establishment of continuous cell lines from embryos of annual killifish Nothobranchius furzeri and N. kadleci
    Tvůrce(i) Součková, K. (CZ)
    Jasík, M. (CZ)
    Sovadinová, I. (CZ)
    Sember, Alexandr (UZFG-Y) RID, ORCID
    Sychrová, E. (CZ)
    Konieczna, A. (PL)
    Bystrý, V. (CZ)
    Dyková, I. (CZ)
    Blažek, Radim (UBO-W) RID, ORCID, SAI
    Lukšíková, Karolína (UZFG-Y)
    Pavlica, Tomáš (UZFG-Y)
    Jankásek, Marek (UZFG-Y)
    Altmanová, Marie (UZFG-Y) ORCID
    Žák, Jakub (UBO-W) RID, ORCID, SAI
    Zbončáková, A. (CZ)
    Reichard, Martin (UBO-W) RID, ORCID, SAI, SAI
    Slabý, O. (CZ)
    Číslo článku106517
    Zdroj.dok.Aquatic Toxicology. - : Elsevier - ISSN 0166-445X
    Roč. 259, June (2023)
    Poč.str.16 s.
    Forma vydáníOnline - E
    Jazyk dok.eng - angličtina
    Země vyd.NL - Nizozemsko
    Klíč. slovakillifish ; embryo ; Nothobranchius ; cell line ; 3-D spheroid ; karyotype
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDGenetics and heredity (medical genetics to be 3)
    CEPGA19-20873S GA ČR - Grantová agentura ČR
    GJ19-22346Y GA ČR - Grantová agentura ČR
    Způsob publikováníOmezený přístup
    Institucionální podporaUZFG-Y - RVO:67985904 ; UBO-W - RVO:68081766
    UT WOS000984669400001
    EID SCOPUS85152737832
    DOI10.1016/j.aquatox.2023.106517
    AnotaceThere is a growing need of alternative experimental models that avoid or minimize the use of animals due to ethical, economical, and scientific reasons. Surprisingly, the stable embryonic cell lines representing Nothobranchius spp., emerging vertebrate models in aging research, regenerative medicine, ecotoxicology, or genomics, have been not derived so far. This paper reports establishment and deep characterization of ten continuous cell lines from annual killifish embryos of N. furzeri and N. kadleci. The established cell lines exhibited mostly fibroblast- and epithelial-like morphology and steady growth rates with cell doubling time ranging from 27 to 40 h. All cell lines retained very similar characteristics even after continuous subcultivation (more than 100 passages) and extended storage in liquid nitrogen (∼3 years). The cytogenetic analysis of the cell lines revealed a diploid chromosome number mostly equal to 38 elements (i.e., the native chromosome count for both killifish species), with minor but diverse line/passage-specific karyotype changes compared to the patterns observed in non-cultured N. furzeri and N. kadleci somatic cells. Based on transcriptional analysis of marker genes, the cell lines displayed features of an undifferentiated state without signs of senescence even in advanced passages. We confirmed that the cell lines are transfectable and can form viable 3-D spheroids. The applicability of the cell lines for (eco)toxicological surveys was confirmed by assessing the effect of cytotoxic and growth inhibitory agents. Properties of established Nothobranchius embryonic cell lines open new possibilities for the application of this model in various fields of life sciences including molecular mechanisms of aging, karyotype (in)stability or differences in lifespan.
    PracovištěÚstav živočišné fyziologie a genetiky
    KontaktJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Rok sběru2024
    Elektronická adresahttps://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=7110796351
Počet záznamů: 1  

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