Počet záznamů: 1
Using the multi-omics approach to reveal the silk composition in Plectrocnemia conspersa
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SYSNO ASEP 0559958 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Using the multi-omics approach to reveal the silk composition in Plectrocnemia conspersa Tvůrce(i) Rouhová, Lenka (BC-A) ORCID
Sehadová, Hana (BC-A) RID, ORCID
Pauchová, Lucie (BC-A)
Hradilová, Miluše (UMG-J)
Žurovcová, Martina (BC-A) RID, ORCID
Šerý, Michal (BC-A) ORCID
Rindoš, Michal (BC-A) ORCID
Žurovec, Michal (BC-A) RID, ORCIDCelkový počet autorů 8 Číslo článku 945239 Zdroj.dok. Frontiers in molecular biosciences
Roč. 9, AUG 11 (2022)Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova fibroin ; zonadhesin ; mucin Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology Vědní obor RIV – spolupráce Ústav molekulární genetiky - Biochemie CEP LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 ; UMG-J - RVO:68378050 UT WOS 000848541300001 EID SCOPUS 85137200525 DOI 10.3389/fmolb.2022.945239 Anotace Similar to Lepidoptera, the larvae of Trichoptera are also capable of producing silk. Plectrocnemia conspersa, a predatory species belonging to the suborder Annulipalpia, builds massive silken retreats with preycapturing nets. In this study, we describe the silk glands of P. conspersa and use the multi-omics methods to obtain a complete picture of the fiber composition. A combination of silk gland- specific transcriptome and proteomic analyses of the spun-out fibers yielded 27 significant candidates whose full-length sequences and gene structures were retrieved from the publicly available genome database. About one-third of the candidates were completely novel proteins for which there are no described homologs, including a group of five pseudofibroins, proteins with a composition similar to fibroin heavy chain. The rest were homologs of lepidopteran silk proteins, although some had a larger number of paralogs. On the other hand, P. conspersa fibers lacked some proteins that are regular components in moth silk. In summary, the multi-omics approach provides an opportunity to compare the overall composition of silk with other insect species. A sufficient number of such studies will make it possible to distinguish between the basic components of all silks and the proteins that represent the adaptation of the fibers for specific purposes or environments. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2023 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmolb.2022.945239/pdf
Počet záznamů: 1