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Transcriptomic markers of fungal growth, respiration and carbon-use efficiency
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SYSNO ASEP 0559317 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transcriptomic markers of fungal growth, respiration and carbon-use efficiency Tvůrce(i) Hasby, F. A. (SE)
Barbi, Florian (MBU-M) ORCID
Manzoni, S. (SE)
Lindahl, B. D. (SE)Číslo článku fnab100 Zdroj.dok. FEMS Microbiology Letters. - : Oxford University Press - ISSN 0378-1097
Roč. 368, č. 15 (2021)Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova growth ; respiration ; carbon-use efficiency ; metatranscriptomics ; gene markers ; fungi Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000692526900008 EID SCOPUS 85114342668 DOI 10.1093/femsle/fnab100 Anotace Fungal metabolic carbon acquisition and its subsequent partitioning between biomass production and respiration, i.e. the carbon-use efficiency (CUE), are central parameters in biogeochemical modeling. However, current available techniques for estimating these parameters are all associated with practical and theoretical shortcomings, making assessments unreliable. Gene expression analyses hold the prospect of phenotype prediction by indirect means, providing new opportunities to obtain information about metabolic priorities. We cultured four different fungal isolates (Chalara longipes, Laccaria bicolor, Serpula lacrymans and Trichoderma harzianum) in liquid media with contrasting nitrogen availability and measured growth rates and respiration to calculate CUE. By relating gene expression markers to measured carbon fluxes, we identified genes coding for 1,3-beta-glucan synthase and 2-oxoglutarate dehydrogenase as suitable markers for growth and respiration, respectively, capturing both intraspecific variation as well as within-strain variation dependent on growth medium. A transcript index based on these markers correlated significantly with differences in CUE between the fungal isolates. Our study paves the way for the use of these markers to assess differences in growth, respiration and CUE in natural fungal communities, using metatranscriptomic or the RT-qPCR approach. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://academic.oup.com/femsle/article/368/15/fnab100/6335482?login=true
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