Počet záznamů: 1
Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation
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SYSNO ASEP 0556763 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Copper Oxide Nanoparticles Stimulate the Immune Response and Decrease Antioxidant Defense in Mice After Six-Week Inhalation Tvůrce(i) Tulinská, J. (SK)
Lehotská Mikušová, M. (SK)
Lisková, A. (SK)
Bušová, M. (CZ)
Mašánová, V. (SK)
Uhnáková, I. (SK)
Rollerová, E. (SK)
Aláčová, R. (SK)
Krivošíková, Z. (SK)
Wsólová, L. (SK)
Dusinská, M. (SK)
Horváthová, M. (SK)
Szabová, E. (SK)
Lukan, N. (SK)
Stuchlíková, M. (SK)
Kuba, D. (SK)
Večeřa, Zbyněk (UIACH-O) RID
Coufalík, Pavel (UIACH-O) ORCID, RID
Křůmal, Kamil (UIACH-O) RID, ORCID
Alexa, Lukáš (UIACH-O) ORCID, RID
Vrlíková, Lucie (UZFG-Y) ORCID
Buchtová, Marcela (UZFG-Y) RID, ORCID
Dumková, J. (CZ)
Piler, P. (CZ)
Thon, V. (CZ)
Mikuška, Pavel (UIACH-O) RID, ORCIDCelkový počet autorů 26 Číslo článku 874253 Zdroj.dok. Frontiers in Immunology. - : Frontiers Media - ISSN 1664-3224
Roč. 13, APR (2022)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova copper oxide nanoparticles ; inhalation ; immune system Vědní obor RIV CB - Analytická chemie, separace Obor OECD Analytical chemistry CEP GBP503/12/G147 GA ČR - Grantová agentura ČR GA20-02203S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UIACH-O - RVO:68081715 ; UZFG-Y - RVO:67985904 UT WOS 000805885900001 EID SCOPUS 85129831954 DOI 10.3389/fimmu.2022.874253 Anotace Copper oxide nanoparticles (CuO NPs) are increasingly used in various industry sectors. Moreover, medical application of CuO NPs as antimicrobials also contributes to human exposure. Their toxicity, including toxicity to the immune system and blood, raises concerns, while information on their immunotoxicity is still very limited. The aim of our work was to evaluate the effects of CuO NPs (number concentration 1.40×106 particles/cm3, geometric mean diameter 20.4 nm) on immune/inflammatory response and antioxidant defense in mice exposed to 32.5 µg CuO/m3 continuously for 6 weeks. After six weeks of CuO NP inhalation, the content of copper in lungs and liver was significantly increased, while in kidneys, spleen, brain, and blood it was similar in exposed and control mice. Inhalation of CuO NPs caused a significant increase in proliferative response of T-lymphocytes after mitogenic stimulation and basal proliferative activity of splenocytes. CuO NPs significantly induced the production of IL-12p70, Th1-cytokine IFN-γ and Th2-cytokines IL-4, IL-5. Levels of TNF-α and IL-6 remained unchanged. Immune assays showed significantly suppressed phagocytic activity of granulocytes and slightly decreased respiratory burst. No significant differences in phagocytosis of monocytes were recorded. The percentage of CD3+, CD3+CD4+, CD3+CD8+, and CD3-CD19+ cell subsets in spleen, thymus, and lymph nodes did not differ between exposed and control animals. No changes in hematological parameters were found between the CuO NP exposed and control groups. The overall antioxidant protection status of the organism was expressed by evaluation of GSH and GSSG concentrations in blood samples. The experimental group exposed to CuO NPs showed a significant decrease in GSH concentration in comparison to the control group. In summary, our results indicate that sub-chronic inhalation of CuO NPs can cause undesired modulation of the immune response. Stimulation of adaptive immunity was indicated by activation of proliferation and secretion functions of lymphocytes. CuO NPs elicited pro-activation state of Th1 and Th2 lymphocytes in exposed mice. Innate immunity was affected by impaired phagocytic activity of granulocytes. Reduced glutathione was significantly decreased in mice exposed to CuO NPs. Pracoviště Ústav analytické chemie Kontakt Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Rok sběru 2023 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fimmu.2022.874253/full
Počet záznamů: 1