Počet záznamů: 1
Ex-vivo Immune-stimulating Activity of Scutellaria baicalensis and Its Major Flavonoids on Human Immune Cells
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SYSNO ASEP 0556002 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Ex-vivo Immune-stimulating Activity of Scutellaria baicalensis and Its Major Flavonoids on Human Immune Cells Tvůrce(i) Vrabec, R. (CZ)
Vokurková, D. (CZ)
Tůmová, L. (CZ)
Cheel Horna, José (MBU-M)Zdroj.dok. Records of Natural Products. - : ACG Publications - ISSN 1307-6167
Roč. 16, č. 2 (2022), s. 188-193Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. TR - Turecko Klíč. slova activation ; leukocytes ; expression ; baicalin ; antigen ; Scutellaria baicalensis ; immune-stimulation ; cd69 ; flavonoids ; baicalin ; baicalein ; wogonoside Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000690403000008 EID SCOPUS 85114016853 DOI 10.25135/rnp.264.21.02.1976 Anotace This study investigates the activation of human immune cells (T, B, and NK cells, and granulocytes) by the ethanolic and aqueous extracts of Scutellaria baicalensis root (SBR) and to identify the compounds responsible for such an effect. The cell activation was determined by expression of the cell surface glycoprotein CD69 (cluster of differentiation 69), as measured by flow cytometry. The content of the main flavonoids in SBR extracts was estimated by high performance liquid chromatography with a diode array detector. NK cells were substantially activated by the SBR ethanolic extract (25-200 mu g/mL), whereas B cells were activated on a lower scale. NK cells were slightly activated by the SBR aqueous extract (100-200 mu g/mL). Wogonoside and baicalin, two major flavonoids present in the SBR extracts, exhibited the highest activation effect on NK cells. The content of baicalin, wogonoside, and baicalein accounted for 201.2, 47.7, and 3.4 mg/g in the ethanolic extract, and for 150.4, 33.6, and 4.6 mg/g in the aqueous extract, respectively. This study shows the immune-stimulating properties of SBR extracts and identifies their active compounds. This data may support the potential utilization of SBR for strengthening the human immune system against bacterial and viral infections. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2023 Elektronická adresa https://www.acgpubs.org/doc/20210825230226A8-264-RNP-2102-1976.pdf
Počet záznamů: 1