Počet záznamů: 1
2,6-diaminopurine promotes repair of DNA lesions under prebiotic conditions
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SYSNO ASEP 0555379 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název 2,6-diaminopurine promotes repair of DNA lesions under prebiotic conditions Tvůrce(i) Szabla, R. (GB)
Zdrowowicz, M. (PL)
Spisz, P. (PL)
Green, N. (GB)
Stadlbauer, Petr (BFU-R) ORCID
Kruse, Holger (BFU-R) ORCID
Šponer, Jiří (BFU-R) RID, ORCID
Rak, J. (PL)Celkový počet autorů 8 Číslo článku 3018 Zdroj.dok. Nature Communications. - : Nature Publishing Group
Roč. 12, č. 1 (2021)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova excited-state dynamics ; cyclobutane pyrimidine dimers ; charge-transfer states ; amber force-field ; molecular-dynamics Obor OECD Biochemistry and molecular biology CEP GA21-23718S GA ČR - Grantová agentura ČR EF15_003/0000477 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000658683500002 EID SCOPUS 85106564592 DOI 10.1038/s41467-021-23300-y Anotace High-yielding and selective prebiotic syntheses of RNA and DNA nucleotides involve UV irradiation to promote the key reaction steps and eradicate biologically irrelevant isomers. While these syntheses were likely enabled by UV-rich prebiotic environment, UV-induced formation of photodamages in polymeric nucleic acids, such as cyclobutane pyrimidine dimers (CPDs), remains the key unresolved issue for the origins of RNA and DNA on Earth. Here, we demonstrate that substitution of adenine with 2,6-diaminopurine enables repair of CPDs with yields reaching 92%. This substantial self-repairing activity originates from excellent electron donating properties of 2,6-diaminopurine in nucleic acid strands. We also show that the deoxyribonucleosides of 2,6-diaminopurine and adenine can be formed under the same prebiotic conditions. Considering that 2,6-diaminopurine was previously shown to increase the rate of nonenzymatic RNA replication, this nucleobase could have played critical roles in the formation of functional and photostable RNA/DNA oligomers in UV-rich prebiotic environments. UV-induced photodamage that likely occurred during the prebiotic synthesis of DNA and RNA is still an untackled issue for their origin on early Earth. Here, the authors show that substitution of 2,6-diaminopurine for adenine enables repair of cyclobutane pyrimidine dimers with high yields, and demonstrate that both 2,6-diaminopurine and adenine nucleosides can be formed under the same prebiotic conditions. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.nature.com/articles/s41467-021-23300-y.pdf
Počet záznamů: 1