Počet záznamů: 1
Gregarine single-cell transcriptomics reveals differential mitochondrial remodeling and adaptation in apicomplexans
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SYSNO ASEP 0554937 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Gregarine single-cell transcriptomics reveals differential mitochondrial remodeling and adaptation in apicomplexans Tvůrce(i) Salomaki, Eric David (BC-A) RID, ORCID
Terpis, K. (US)
Rueckert, S. (GB)
Kotyk, M. (CZ)
Varadinová, Z. (CZ)
Čepička, I. (CZ)
Lane, C. E. (US)
Kolísko, Martin (BC-A) ORCIDCelkový počet autorů 8 Číslo článku 77 Zdroj.dok. BMC BIOLOGY. - : BioMed Central
Roč. 19, č. 1 (2021)Poč.str. 19 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova horizontal gene-transfer ; cytoplasmic malate-dehydrogenases ; acetyl-coa synthetase ; citric-acid cycle ; genome-sequence ; pyruvate-nadp(+) oxidoreductase ; cryptosporidium-parvum ; energy-metabolism ; rodent malaria ; atp synthase ; Mitochondria ; Apicomplexa ; Anaerobic metabolism ; Phylogenomics ; Evolution ; Eugregarines ; Mitosome ; Parasitism Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP EF16_027/0008357 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF20_079/0017809 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA19-19297S GA ČR - Grantová agentura ČR GA18-28103S GA ČR - Grantová agentura ČR EF16_019/0000759 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000641666500001 EID SCOPUS 85104444682 DOI 10.1186/s12915-021-01007-2 Anotace BackgroundApicomplexa is a diverse phylum comprising unicellular endobiotic animal parasites and contains some of the most well-studied microbial eukaryotes including the devastating human pathogens Plasmodium falciparum and Cryptosporidium hominis. In contrast, data on the invertebrate-infecting gregarines remains sparse and their evolutionary relationship to other apicomplexans remains obscure. Most apicomplexans retain a highly modified plastid, while their mitochondria remain metabolically conserved. Cryptosporidium spp. inhabit an anaerobic host-gut environment and represent the known exception, having completely lost their plastid while retaining an extremely reduced mitochondrion that has lost its genome. Recent advances in single-cell sequencing have enabled the first broad genome-scale explorations of gregarines, providing evidence of differential plastid retention throughout the group. However, little is known about the retention and metabolic capacity of gregarine mitochondria.ResultsHere, we sequenced transcriptomes from five species of gregarines isolated from cockroaches. We combined these data with those from other apicomplexans, performed detailed phylogenomic analyses, and characterized their mitochondrial metabolism. Our results support the placement of Cryptosporidium as the earliest diverging lineage of apicomplexans, which impacts our interpretation of evolutionary events within the phylum. By mapping in silico predictions of core mitochondrial pathways onto our phylogeny, we identified convergently reduced mitochondria. These data show that the electron transport chain has been independently lost three times across the phylum, twice within gregarines.ConclusionsApicomplexan lineages show variable functional restructuring of mitochondrial metabolism that appears to have been driven by adaptations to parasitism and anaerobiosis. Our findings indicate that apicomplexans are rife with convergent adaptations, with shared features including morphology, energy metabolism, and intracellularity. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-01007-2
Počet záznamů: 1