Počet záznamů: 1
Nitro-Oleic Acid Inhibits Stemness Maintenance and Enhances Neural Differentiation of Mouse Embryonic Stem Cells via STAT3 Signaling
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SYSNO ASEP 0554491 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nitro-Oleic Acid Inhibits Stemness Maintenance and Enhances Neural Differentiation of Mouse Embryonic Stem Cells via STAT3 Signaling Tvůrce(i) Perečková, Jana (BFU-R) ORCID
Pekarová, Michaela (BFU-R) RID
Szamecová, Nikoletta (BFU-R)
Hoferová, Zuzana (BFU-R) RID
Kamarýtov, Kristýna (BFU-R)
Falk, Martin (BFU-R) RID, ORCID
Perečko, Tomáš (BFU-R) RID, ORCIDCelkový počet autorů 7 Číslo článku 9981 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 18 (2021)Poč.str. 12 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova self-renewal ; fatty-acids ; pluripotency ; cardiomyogenesis ; neurons ; target ; nitro-oleic acid Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GJ17-08066Y GA ČR - Grantová agentura ČR LTAUSA17160 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000699693400001 EID SCOPUS 85114871373 DOI 10.3390/ijms22189981 Anotace Nitro-oleic acid (NO2-OA), pluripotent cell-signaling mediator, was recently described as a modulator of the signal transducer and activator of transcription 3 (STAT3) activity. In our study, we discovered new aspects of NO2-OA involvement in the regulation of stem cell pluripotency and differentiation. Murine embryonic stem cells (mESC) or mESC-derived embryoid bodies (EBs) were exposed to NO2-OA or oleic acid (OA) for selected time periods. Our results showed that NO2-OA but not OA caused the loss of pluripotency of mESC cultivated in leukemia inhibitory factor (LIF) rich medium via the decrease of pluripotency markers (NANOG, sex-determining region Y-box 1 transcription factor (SOX2), and octamer-binding transcription factor 4 (OCT4)). The effects of NO2-OA on mESC correlated with reduced phosphorylation of STAT3. Subsequent differentiation led to an increase of the ectodermal marker orthodenticle homolog 2 (Otx2). Similarly, treatment of mESC-derived EBs by NO2-OA resulted in the up-regulation of both neural markers Nestin and beta-Tubulin class III (Tubb3). Interestingly, the expression of cardiac-specific genes and beating of EBs were significantly decreased. In conclusion, NO2-OA is able to modulate pluripotency of mESC via the regulation of STAT3 phosphorylation. Further, it attenuates cardiac differentiation on the one hand, and on the other hand, it directs mESC into neural fate. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/1422-0067/22/18/9981
Počet záznamů: 1