Počet záznamů: 1
Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress
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SYSNO ASEP 0546175 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Priming maritime pine megagametophytes during somatic embryogenesis improved plant adaptation to heat stress Tvůrce(i) Pérez-Oliver, M. A. (ES)
Haro, J. G. (ES)
Pavlović, Iva (UEB-Q) ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Segura, J. (ES)
Sales, E. (ES)
Arrillaga, I. (ES)Celkový počet autorů 7 Číslo článku 446 Zdroj.dok. Plants. - : MDPI
Roč. 10, č. 3 (2021)Poč.str. 24 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Heat stress ; Hormones ; hsp ; Photosynthesis ; Pinus pinaster ; Priming ; ros ; Somatic embryogenesis ; Transgenerational memory ; wrky Obor OECD Plant sciences, botany CEP GA20-22875S GA ČR - Grantová agentura ČR EF16_019/0000827 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UEB-Q - RVO:61389030 UT WOS 000634069800001 EID SCOPUS 85101632269 DOI 10.3390/plants10030446 Anotace In the context of global climate change, forest tree research should be addressed to provide genotypes with increased resilience to high temperature events. These improved plants can be obtained by heat priming during somatic embryogenesis (SE), which would produce an epigenetic-mediated transgenerational memory. Thereby, we applied 37 °C or 50 °C to maritime pine (Pinus pinaster) megagametophytes and the obtained embryogenic masses went through the subsequent SE phases to produce plants that were further subjected to heat stress conditions. A putative transcription factor WRKY11 was upregulated in priming-derived embryonal masses, and also in the regenerated P37 and P50 plants, suggesting its role in establishing an epigenetic memory in this plant species. In vitro-grown P50 plants also showed higher cytokinin content and SOD upregula-tion, which points to a better responsiveness to heat stress. Heat exposure of two-year-old maritime pine plants induced upregulation of HSP70 in those derived from primed embryogenic masses, that also showed better osmotic adjustment and higher increases in chlorophyll, soluble sugars and starch contents. Moreover, ɸPSII of P50 plants was less affected by heat exposure. Thus, our results suggest that priming at 50 °C at the SE induction phase is a promising strategy to improve heat resilience in maritime pine. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2022 Elektronická adresa http://doi.org/10.3390/plants10030446
Počet záznamů: 1