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The potential of SERS as an AST methodology in clinical settings
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SYSNO ASEP 0544143 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The potential of SERS as an AST methodology in clinical settings Tvůrce(i) Samek, Ota (UPT-D) RID, ORCID, SAI
Bernatová, Silvie (UPT-D) RID, SAI
Dohnal, F. (AT)Celkový počet autorů 3 Zdroj.dok. Nanophotonics . - : Walter de Gruyter - ISSN 2192-8606
Roč. 10, č. 10 (2021), s. 2537-2561Poč.str. 25 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova biosensor ; infection disease management ; surface enhanced Raman scattering ; vibration spectroscopy Vědní obor RIV BH - Optika, masery a lasery Obor OECD Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect) CEP GA19-20697S GA ČR - Grantová agentura ČR GF19-29651L GA ČR - Grantová agentura ČR LO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UPT-D - RVO:68081731 UT WOS 000686355800001 EID SCOPUS 85110322159 DOI 10.1515/nanoph-2021-0095 Anotace The ability to identify and characterize microorganisms from tiny sample volumes in a rapid and reliable way is the first and crucial step in the diagnostics of microbial infections. Ideal analytical techniques would require minimal and low-cost sample preparation, permit automatic analysis of many serial samples, and allow rapid classification of present microorganisms against a stable database. Current practice, however, is far from this ideal, a typical analytical procedure might require a few days. Delayed laboratory results might lead, for example, to progress/spread of the infection, more serious condition of the patient, even death, prescription of inappropriate antibiotics that could be ineffective against causative agents and may as well contribute to the emerging problem of drug resistance in microorganisms. Several studies confirmed that surface enhanced Raman scattering (SERS) is capable of a rapid identification and discrimination of biological samples including medically relevant bacteria. A typical spectrum contains a wealth of information indicative of the cellular content of nucleic acids, purine bases, proteins, carbohydrates, and lipids. Such a spectrum functions as a cellular 'fingerprint' and serves as a sensitive indicator of the physiological state of the cell which in turn enables to differentiate cell types, actual physiological states, nutrient conditions, and phenotype changes. Consequently, the focus of this review is on the SERS spectra of bacteria which result from secreted metabolic substances the purine bases which are a common feature in the label-free SERS research related to clinical diagnostics of pathogens. Here is the review of the current status of SERS applications on bacteria. A special attention is given to the efforts of profiling antimicrobial susceptibility at clinically relevant species, which in turn has a great potential for use in routine point-of-care (POC) tests. Thus, early and accurate infection disease management can be provided at the bedside or at remote care centres. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2022 Elektronická adresa https://www.degruyter.com/document/doi/10.1515/nanoph-2021-0095/html
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