Počet záznamů: 1
Fibroblast Activation Protein Expressing Mesenchymal Cells Promote Glioblastoma Angiogenesis
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SYSNO ASEP 0544017 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fibroblast Activation Protein Expressing Mesenchymal Cells Promote Glioblastoma Angiogenesis Tvůrce(i) Balážiová, E. (CZ)
Výmola, P. (CZ)
Hrabal, P. (CZ)
Mateu, R. (CZ)
Zubal, M. (CZ)
Tomáš, R. (CZ)
Netuka, D. (CZ)
Kramář, F. (CZ)
Zemanová, Z. (CZ)
Svobodová, K. (CZ)
Brabec, Marek (UIVT-O) RID, SAI, ORCID
Šedo, A. (CZ)
Bušek, P. (CZ)Číslo článku 3304 Zdroj.dok. Cancers (Basel). - : MDPI
Roč. 13, č. 13 (2021)Poč.str. 24 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova glioblastoma ; angiogenesis ; microenvironment ; fibroblast activation protein ; seprase ; angiopoietin ; vessel destabilisation Vědní obor RIV BB - Aplikovaná statistika, operační výzkum Obor OECD Statistics and probability CEP NU21-04-00100 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora UIVT-O - RVO:67985807 UT WOS 000670920100001 EID SCOPUS 85108891582 DOI 10.3390/cancers13133304 Anotace Fibroblast activation protein (FAP) is a membrane-bound protease that is upregulated in a wide range of tumours and viewed as a marker of tumour-promoting stroma. Previously, we demonstrated increased FAP expression in glioblastomas and described its localisation in cancer and stromal cells. In this study, we show that FAP+ stromal cells are mostly localised in the vicinity of activated CD105+ endothelial cells and their quantity positively correlates with glioblastoma vascularisation. FAP+ mesenchymal cells derived from human glioblastomas are non-tumorigenic and mostly lack the cytogenetic aberrations characteristic of glioblastomas. Conditioned media from these cells induce angiogenic sprouting and chemotaxis of endothelial cells and promote migration and growth of glioma cells. In a chorioallantoic membrane assay, co-application of FAP+ mesenchymal cells with glioma cells was associated with enhanced abnormal angiogenesis, as evidenced by an increased number of erythrocytes in vessel-like structures and higher occurrence of haemorrhages. FAP+ mesenchymal cells express proangiogenic factors, but in comparison to normal pericytes exhibit decreased levels of antiangiogenic molecules and an increased Angiopoietin 2/1 ratio. Our results show that FAP+ mesenchymal cells promote angiogenesis and glioma cell migration and growth by paracrine communication and in this manner, they may thus contribute to glioblastoma progression. Pracoviště Ústav informatiky Kontakt Tereza Šírová, sirova@cs.cas.cz, Tel.: 266 053 800 Rok sběru 2022 Elektronická adresa http://dx.doi.org/10.3390/cancers13133304
Počet záznamů: 1