Počet záznamů: 1
Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis
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SYSNO ASEP 0541492 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effects of DNA Topology on Transcription from rRNA Promoters in Bacillus subtilis Tvůrce(i) Sudzinová, Petra (MBU-M) ORCID
Kambová, Milada (MBU-M)
Ramaniuk, Olga (MBU-M) ORCID
Benda, Martin (MBU-M) ORCID
Šanderová, Hana (MBU-M) RID, ORCID
Krásný, Libor (MBU-M) RID, ORCIDČíslo článku 87 Zdroj.dok. Microorganisms. - : MDPI
Roč. 9, č. 1 (2021)Poč.str. 17 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Bacillus subtilis ; transcription ; ribosomal RNA ; DNA topology Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA20-12109S GA ČR - Grantová agentura ČR Výzkumná infrastruktura C4SYS - 90055 - Mikrobiologický ústav AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000610593600001 EID SCOPUS 85099025032 DOI 10.3390/microorganisms9010087 Anotace The expression of rRNA is one of the most energetically demanding cellular processes and, as such, it must be stringently controlled. Here, we report that DNA topology, i.e., the level of DNA supercoiling, plays a role in the regulation of Bacillus subtilis sigma(A)-dependent rRNA promoters in a growth phase-dependent manner. The more negative DNA supercoiling in exponential phase stimulates transcription from rRNA promoters, and DNA relaxation in stationary phase contributes to cessation of their activity. Novobiocin treatment of B. subtilis cells relaxes DNA and decreases rRNA promoter activity despite an increase in the GTP level, a known positive regulator of B. subtilis rRNA promoters. Comparative analyses of steps during transcription initiation then reveal differences between rRNA promoters and a control promoter, Pveg, whose activity is less affected by changes in supercoiling. Additional data then show that DNA relaxation decreases transcription also from promoters dependent on alternative sigma factors sigma(B), sigma(D), sigma(E), sigma(F), and sigma(H) with the exception of sigma(N) where the trend is the opposite. To summarize, this study identifies DNA topology as a factor important (i) for the expression of rRNA in B. subtilis in response to nutrient availability in the environment, and (ii) for transcription activities of B. subtilis RNAP holoenzymes containing alternative sigma factors. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2076-2607/9/1/87
Počet záznamů: 1